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HomeProduct name listDisodium 4-nitrophenylphosphate

Disodium 4-nitrophenylphosphate

Synonym(s):p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS 4264-83-9 - Calbiochem;p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem;pNPP, 2Na;4-Nitrophenyl phosphate disodium salt solution;pNPP

  • CAS NO.:4264-83-9
  • Empirical Formula: C6H7NNaO6P
  • Molecular Weight: 243.09
  • MDL number: MFCD00007319
  • EINECS: 224-246-5
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-30 18:52:02
Disodium 4-nitrophenylphosphate Structural

What is Disodium 4-nitrophenylphosphate?

Chemical properties

White crystalline

The Uses of Disodium 4-nitrophenylphosphate

PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.

What are the applications of Application

PNPP, Disodium Salt is an alkaline phosphatase substrate used in ELISA applications while absorbing light at 405nm.

General Description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.

Biochem/physiol Actions

Primary TargetSubstrate for phosphatase

Purification Methods

Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]

Properties of Disodium 4-nitrophenylphosphate

Melting point: >300 °C(lit.)
Density  1.751 at 20℃
vapor pressure  15Pa at 20-50℃
storage temp.  -20°C
solubility  H2O: 1 tablet/3 mL 25 mM, clear, light yellow
form  tablets
color  white to pale yellow
Water Solubility  Miscible with water. Immiscible with ethanol and acetone.
BRN  5324661
Stability: Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.
CAS DataBase Reference 4264-83-9(CAS DataBase Reference)
EPA Substance Registry System Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9)

Safety information for Disodium 4-nitrophenylphosphate

Signal word Danger
Pictogram(s)
ghs
Corrosion
Corrosives
GHS05
ghs
Health Hazard
GHS08
GHS Hazard Statements H290:Corrosive to Metals
H315:Skin corrosion/irritation
H318:Serious eye damage/eye irritation
H373:Specific target organ toxicity, repeated exposure
Precautionary Statement Codes P202:Do not handle until all safety precautions have been read and understood.
P234:Keep only in original container.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352:IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P308+P313:IF exposed or concerned: Get medical advice/attention.

Computed Descriptors for Disodium 4-nitrophenylphosphate

InChIKey KAKKHKRHCKCAGH-UHFFFAOYSA-L

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