Disodium 4-nitrophenylphosphate
Synonym(s):p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS 4264-83-9 - Calbiochem;p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem;pNPP, 2Na;4-Nitrophenyl phosphate disodium salt solution;pNPP
- CAS NO.：4264-83-9
- Empirical Formula: C6H7NNaO6P
- Molecular Weight: 243.09
- MDL number: MFCD00007319
- EINECS: 224-246-5
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-21 18:03:03
What is Disodium 4-nitrophenylphosphate?
Chemical properties
White crystalline
The Uses of Disodium 4-nitrophenylphosphate
PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.
What are the applications of Application
PNPP, Disodium Salt is an alkaline phosphatase substrate used in ELISA applications while absorbing light at 405nm.
General Description
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.
Biochem/physiol Actions
Primary TargetSubstrate for phosphatase
Purification Methods
Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]
Properties of Disodium 4-nitrophenylphosphate
| Melting point: | >300 °C(lit.) |
| Density | 1.751 at 20℃ |
| vapor pressure | 15Pa at 20-50℃ |
| storage temp. | -20°C |
| solubility | H2O: 1 tablet/3 mL 25 mM, clear, light yellow |
| form | tablets |
| color | white to pale yellow |
| Water Solubility | Miscible with water. Immiscible with ethanol and acetone. |
| BRN | 5324661 |
| Stability: | Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents. |
| CAS DataBase Reference | 4264-83-9(CAS DataBase Reference) |
| EPA Substance Registry System | Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9) |
Safety information for Disodium 4-nitrophenylphosphate
| Signal word | Danger |
| Pictogram(s) |
 Corrosion Corrosives GHS05  Health Hazard GHS08 |
| GHS Hazard Statements |
H290:Corrosive to Metals H315:Skin corrosion/irritation H318:Serious eye damage/eye irritation H373:Specific target organ toxicity, repeated exposure |
| Precautionary Statement Codes |
P202:Do not handle until all safety precautions have been read and understood. P234:Keep only in original container. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P308+P313:IF exposed or concerned: Get medical advice/attention. |
Computed Descriptors for Disodium 4-nitrophenylphosphate
| InChIKey | KAKKHKRHCKCAGH-UHFFFAOYSA-L |
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