3,5-Dinitrosalicylic acid

Synonym(s):2-Hydroxy-3,5-dinitrobenzoic acid;3,5-Dinitro-2-hydroxybenzoic acid;DNS

CAS NO.：609-99-4
Empirical Formula: C7H4N2O7
Molecular Weight: 228.12
MDL number: MFCD00149501
EINECS: 210-204-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-12-18 14:15:32

What is 3,5-Dinitrosalicylic acid?

Chemical properties

solid

The Uses of 3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars.

The Uses of 3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid is used in the quantification of reducing sugars by colorimetry. It is utilized for the analysis of glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. It is also used to detect reducing substances in urine and for quantifying carbohydrate level in blood. It acts as an important raw material and an intermediate in the production of organic chemicals such as agrochemicals, organic synthesis, pharmaceuticals and dyes.

The Uses of 3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars.

What are the applications of Application

3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars

Definition

ChEBI: A monohydroxybenzoic acid consisting of 2-hydroxybenzoic acid having nitro substituents at the 3- and 5-positions. It is used in colorimetric testing for the presence of free carbonyl groups (C2O) in reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Purification Methods

Crystallise the acid from H2O. [Beilstein 10 IV 270.]

Properties of 3,5-Dinitrosalicylic acid

Melting point:	168-172 °C (lit.)
Boiling point:	369.91°C (rough estimate)
Density	1.7914 (rough estimate)
refractive index	1.5300 (estimate)
storage temp.	room temp
solubility	H2O: soluble50mg/mL
pka	1.57±0.10(Predicted)
form	Powder
color	Yellow
PH Range	1.3-1.8 (10g/L @ 20°C)
Water Solubility	soluble
BRN	2220661
Stability:	Stable. Incompatible with strong oxidizing agents, strong bases, strong acids.
CAS DataBase Reference	609-99-4(CAS DataBase Reference)
NIST Chemistry Reference	3,5-Dinitrosalicylic acid(609-99-4)
EPA Substance Registry System	Benzoic acid, 2-hydroxy-3,5-dinitro- (609-99-4)

Safety information for 3,5-Dinitrosalicylic acid

Signal word	Danger
Pictogram(s)	Flame Flammables GHS02 Exclamation Mark Irritant GHS07
GHS Hazard Statements	H228:Flammable solids H302:Acute toxicity,oral
Precautionary Statement Codes	P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P240:Ground/bond container and receiving equipment. P241:Use explosion-proof electrical/ventilating/lighting/…/equipment. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P270:Do not eat, drink or smoke when using this product. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.