MES sodium salt

Synonym(s):2-(N-Morpholino)ethanesulfonic acid sodium salt;4-Morpholineethanesulfonic acid sodium salt

CAS NO.：71119-23-8
Empirical Formula: C6H14NNaO4S
Molecular Weight: 219.23
MDL number: MFCD00065473
EINECS: 275-203-2
SAFETY DATA SHEET (SDS)
Update Date: 2024-12-18 14:15:32

What is MES sodium salt?

Chemical properties

White powder

The Uses of MES sodium salt

MES sodium salt is a buffering agent used in biological and biochemical research including plant cell culture. It has been used:
To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA.
To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization.

The Uses of MES sodium salt

MES Sodium is a biological buffer often referred to as a “Good′s” buffer. The pKa of MES is 5.96 which makes MES an ideal candidate for cell culture media and protein based buffer formulations to maintain a stable environment in solution. MES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

MES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. MES based buffers are used in purification bioprocesses of antibodies, peptides, proteins and blood components.

What are the applications of Application

MES, Sodium Salt is a buffering agent used in biological and biochemical research including plant cell culture

Definition

ChEBI: Sodium 2-(N-morpholino)ethanesulfonate is the organic sodium salt that is the sodium salt of 2-(N-morpholino)ethanesulfonic acid. It contains a 2-(N-morpholino)ethanesulfonate.

Preparation

The amine starting material, aqueous sodium 2-hydroxyethanesulfonate, and aqueous 50 percent sodium hydroxide were charged to the autoclave for reaction. After purification, MES sodium salt can be obtained.

General Description

MES is one of the so-called "Good" buffers developed for biological applications. This product is BioPerformance certified (suitable for electrophoresis, tissue culture, and molecular biology applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Flammability and Explosibility

Not classified

Properties of MES sodium salt

Melting point:	>250?C (dec.)
Density	1.507[at 20℃]
vapor pressure	0Pa at 25℃
storage temp.	room temp
solubility	H₂O: 0.5 g/mL, clear, colorless
form	Powder
color	White
PH Range	5.5 - 6.7
Water Solubility	335.3g/L at 20℃
BRN	3765682
InChI	InChI=1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
CAS DataBase Reference	71119-23-8(CAS DataBase Reference)
EPA Substance Registry System	4-Morpholineethanesulfonic acid, sodium salt (71119-23-8)

Safety information for MES sodium salt

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.