Girard's Reagent T

Synonym(s):(Carboxymethyl)trimethylammonium chloride hydrazide;(Hydrazinocarbonylmethyl)trimethylammonium chloride;Acethydrazide trimethylammonium chloride

CAS NO.：123-46-6
Empirical Formula: C5H14N3O.Cl
Molecular Weight: 167.64
MDL number: MFCD00012009
EINECS: 204-629-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-08-15 19:00:21

What is Girard's Reagent T?

Chemical properties

white to almost white crystalline powder

The Uses of Girard's Reagent T

Girard''s Reagent T is a derivatizing agent used to generate permanent cationic charge at the reducing ends of neutral oligosaccharides for easier quantification in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

The Uses of Girard's Reagent T

Girard′s reagent T may be used as a derivatizing reagent for the determination of 5-Formyl-2′-deoxyuridine in cellular DNA samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique. Reducing saccharides can be derivatized by Girard′s reagent T and this can be analyzed by matrix-assisted laser desorption/ionization coupled to time of flight mass spectrometry (MALDI-TOF-MS) technique.

Purification Methods

It is purified by crystallisation from absolute EtOH (slight decomposition) until it has only a slight odour. Store it in well-stoppered containers because it is very hygroscopic. It is very soluble in H2O, AcOH and glycerol but slightly soluble in EtOH (0.66%). It forms water-soluble hydrazones with carbonyl compounds. [Beilstein 4 III 1133.]

Properties of Girard's Reagent T

Melting point:	188-192 °C (dec.) (lit.)
Density	1.3799 (rough estimate)
refractive index	1.5400 (estimate)
storage temp.	Inert atmosphere,Room Temperature
form	powder to crystal
color	White to Almost white
Water Solubility	soluble
Merck	14,4429
BRN	3727163
CAS DataBase Reference	123-46-6(CAS DataBase Reference)
EPA Substance Registry System	Ethanaminium, 2-hydrazino-N,N,N-trimethyl-2-oxo-, chloride (123-46-6)

Safety information for Girard's Reagent T

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention.