DANSYLCADAVERINE

Synonym(s):N-(5-Amino-pentyl)-5-di-methyl-amino-naphtha-lene-1-sulfon-amide;N-(Dimethyl-amino-naphtha-lene-sulfonyl)-1,5-pentane-diamine;Monodansyl cadaverine

CAS NO.：10121-91-2
Empirical Formula: C17H25N3O2S
Molecular Weight: 335.46
MDL number: MFCD00042704
EINECS: 233-326-9
SAFETY DATA SHEET (SDS)
Update Date: 2023-06-08 09:02:15

What is DANSYLCADAVERINE?

Description

Monodansylcadaverine (MDC) is a fluorescent marker for autophagic vacuoles. It is an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and interaction with membrane lipids, exhibiting a Stokes shift and increased relative fluorescence in hydrophobic environments. MDC selectively accumulates in PaTu 8902 subcellular fractions containing the lysosomal enzymes acid phosphatase and cathepsin D, but not those containing the rough and smooth endoplasmic reticulum markers TRAM and cytochrome P450, respectively.

The Uses of DANSYLCADAVERINE

Dansylcadaverine is a fluorescent amine probe, which is believed to directly inhibit vesicular transport. Studies indicate that Dansylcadaverine can act as a marker used to study autophagy in the cytoplasm and vacuoles of plants and mammalian cells.

The Uses of DANSYLCADAVERINE

Fluorescent substrate for the assay of transamidating enzymes.

The Uses of DANSYLCADAVERINE

Lysosomotropic fluorescent Probe

What are the applications of Application

Dansylcadaverine is a fluorescent amine probe

Definition

ChEBI: Monodansylcadaverine is a sulfonamide obtained by formal condensation of the sulfo group of 5-(dimethylamino)naphthalene-1-sulfonic acid with one of the amino groups of cadaverine. It has a role as a fluorochrome, a protective agent and an EC 2.3.2.13 (protein-glutamine gamma-glutamyltransferase) inhibitor. It is a sulfonamide, an aminonaphthalene, a tertiary amino compound and a primary amino compound. It is functionally related to a cadaverine.

References

1) Munafo and Colombo (2001),?A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation; Int. J. Cell Sci.,?114?3619 2) Jiang?et al. (2012),?Targeting androgen receptor leads to suppression of prostate cancer via induction of autophagy; J. Urol.,?188?1361 3) Haigler?et al.?(1980),?Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells; J. Biol. Chem., 255 1239 4) Murthy?et al. (1994),?Residue Gln-30 of human erythrocyte anion transporter is a prime site for reaction with intrinsic transglutaminase; J. Biol. Chem.,?269?22907

Properties of DANSYLCADAVERINE

Melting point:	137-140 °C
Boiling point:	505.5±60.0 °C(Predicted)
Density	1.190±0.06 g/cm3(Predicted)
storage temp.	−20°C
solubility	Do you have solubility information on this product that you would like to share
form	powder
pka	11.96±0.50(Predicted)
color	Yellow to yellowish green
λmax	335 nm
BRN	2951070
Stability:	Stable for 1 year from date of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
Biological Applications	Measuring cardiac autophagic flux; treating cataract,fibrosis,Parkinson’s disease,peritoneal ovarian tumor dissemination,respiratory diseases,lung diseases,Huntington’s disease,spinobulbar atrophy,spinocerebellar ataxia,denta

Safety information for DANSYLCADAVERINE

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.