Thioflavin T

Chemical properties

yellow to ochre-yellow powder

The Uses of Thioflavin T

Textile dyeing, fluorescent sign paints, in combination with green or blue pigments to produce brilliant greens, phosphotungstic pigments.

The Uses of Thioflavin T

A dye used to visualize beta-amyloid plaques, like those associated with Alzheimer's Disease

What are the applications of Application

Thioflavin T is a cell-permeable dye capable of binding amyloid fibrils

Preparation

4-(6-Methyl-2,3-dihydrobenzo[d]thiazol-2-yl)benzenamine?with methanol and hydrochloric acid or sulfuric acid, at 160 ~ 170 ℃ methylation under pressure.

Definition

ChEBI: Thioflavine T is an organic chloride salt having 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium as the counterion. It is widely used to visualise and quantify the presence of amyloids, both in vitro and in vivo. It has a role as a fluorochrome, a histological dye and a geroprotector. It contains a thioflavin T cation.

General Description

Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid. Thioflavin T has been used in histology and for protein characterization.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

Purification Methods

Crystallise the chloride from *benzene/EtOH (1:1). [Beilstein 27 III/IV 5052.]

Properties and Applications

colourful green light yellow. Soluble in cold water, soluble in hot water for yellow, easily soluble in ethanol and yellow, with green fluorescent. The strong sulfuric acid to colorless, a green fluorescent, diluted into light yellow.