Sodium 2-propylpentanoate

Description

Sodium Valproate (1069-66-5) is a histone deacetylase inhibitor (IC50 = 400μM).1 Demonstrates neuroprotective, anticancer, and anti-inflammatory activity.2? Inhibits Aβ production, reduced neuritic plaque formation, and improved memory deficits in Alzheimer’s mouse models.3? Improves stem cell reprogramming efficiency and enables efficient induction of pluripotency without introduction of the oncogene c-Myc.4 Clinically useful anticonvulsant.

Chemical properties

White Solid. Colorless crystalline powder, hygroscopic, very soluble in water (ca. 0.66 g/mL of solution), soluble in ethanol (ca. 0.2 g/mL of solution), practically insoluble in chloroform and diethyl ether.

Originator

Anticon,Generics-UK,UK

The Uses of Sodium 2-propylpentanoate

Antiepileptic; increases levels of GABA in the brain

The Uses of Sodium 2-propylpentanoate

antibacterial

The Uses of Sodium 2-propylpentanoate

Anticonvulsant

What are the applications of Application

Valproic acid sodium salt is an HDAC inhibitor with anticancer, anti-inflammatory and neuroprotective effects

Definition

ChEBI: The sodium salt of valproic acid.

Manufacturing Process

(a) Di-n-propyl cyanacetic acid First of all, a sodium n-propylate solution was prepared from 7.42 g (0.322 mol) of sodium and 180 ml of anhydrous n-propanol, by heating with gentle reflux until complete dissolution of the sodium.
Into a 500 ml spherical flask, equipped with a dropping funnel, a mechanical stirrer, a thermometer and a condenser, above which was disposed a calcium chloride trap, were introduced 16.95 g (0.141 mol) of ethyl cyanacetate and 40.69 g (0.33 mol) of n-propyl bromide. This mixture was heated to 45°C and then there was added thereto, slowly and while stirring, the previously prepared solution of sodium n-propylate, keeping the temperature of the reaction medium at 50°-55°C by gentle external cooling.With the completion of the operation of introduction, the mixture was brought to reflux temperature in 30 minutes and kept at this temperature for 3 hours. The n-propanol was then distilled and the distillation stopped when the temperature of the residual mass had reached 115°C.
The crude ester obtained in this way was then treated with a solution of 7.5 g of flaked sodium hydroxide in 67.5 ml of water. The mixture was introduced into a 250 ml spherical flask, equipped with a condenser, and then the reaction medium was slowly brought to 60°-70°C. This temperature was maintained for 3 hours, whereafter the mixture was cooled to about 50°C and the ethanol which had formed and the residue of n-propanol were eliminated under a pressure of 70 mm Hg. The solution thus obtained was cooled to 20°C and acidified, while stirring, by addition of 26.25 g of 36% hydrochloric acid. During this operation, the temperature of the reaction medium was kept below 40°C by cooling. Stirring was continued for 30 minutes, whereafter the mixture was left standing for 30 minutes. The oily layer of di-n-propyl cyanacetic acid was decanted and the aqueous phase extracted with 35 ml of toluene. The extract in toluene was then added to the decanted di-n-propyl cyanacetic acid, whereafter the solution in toluene was washed, in a separation funnel, with a solution of 1.5 g of sodium chloride in 14 ml of water. The toluenic phase was decanted and the toluene distilled under atmospheric pressure.
Using this procedure, 25 g of crude di-n-propyl cyanacetic acid were obtained. (b) Di-n-propyl acetonitrile Into a 100 ml spherical flask fitted with a thermometer and a condenser were introduced 25 g of crude di-n-propyl cyanacetic acid obtained by the method previously described, and the mixture was heated on an oil bath. Decarboxylation commenced at a temperature in the region of 140°C. The mixture was refluxed at about 160°C and at 190°C for 2 hours. This temperature was maintained until the release of gas was completed, this taking 2 hours. The di-n-propyl acetonitrile thus formed was then slowly distilled and the fraction passing over between 165°C and 175°C was collected. A second distillation was then carried out. Using this procedure, 14.7 g of di-n-propyl acetonitrile were collected. Boiling point: 170°C. Yield: 83%, relatively to the ethyl cyanacetate used. Di-n-propyl acetonitrile may be saponifyed with equal molecular quantity of NaOH to give the desired valproic acid (valproate). After that it may be converted into the sodium salt with help of equivalent NaOH to give the valproate sodium.

brand name

Convulex (Byk – Gulden, Promonta Lundbeck), Depakene/Depakote/ Valcote (Abbott), Depakine/Epilim/Ergenyl (Sanofi), Leptilan (Novartis), Orfiril (Desitin Arzneimittel)

Therapeutic Function

Anticonvulsant, Antiepileptic

General Description

A cell-permeable, short-chained fatty acid that inhibits histone deacetylase activity (IC₅₀ = 400 μM for HDAC1). Induces differentiation and inhibits proliferation of cell lines derived from human malignant gliomas. At therapeutic levels (350 μM-1.04 mM), causes inositol depletion, inhibits both GSK-3α and -3β, activates the ERK pathway, and produces neurotropic effects. Has been used as an anti-epileptic agent. Also reported to stimulate peroxisome proliferator-activated receptor (PPAR) activity. Displays a potent teratogenic activity in humans and rodent models.

Biological Activity

Histone deacetylase inhibitor (IC 50 = 400 μ M) that exhibits anticancer, anti-inflammatory and neuroprotective effects. Displays anticonvulsive activity via an increase in GABA levels and decreases A β production in animal models of Alzheimer's disease. Also attenuates NMDA-mediated excitation, blocks voltage-gated Na + channels and modulates firing of neurons. Enables induction of pluripotent stem cells from somatic cells by Oct4 and Sox2.

Biochem/physiol Actions

Cell permeable: yes

Clinical Use

All forms of epilepsy Migraine prophylaxis (unlicensed)

in vitro

vpa showed to have cellular neuroprotective properties. in cultured neurons, vpa protected from thapsigargin-induced endoplasmic reticulum stress, glutamate-induced excitotoxicity, as well as lipopolysaccharide (lps)-induced dopaminergic neuronal death. in midbrain neuron-glia cultures, vpa was also shown to inhibit lps-induced, microglia-mediated inflammation [1].

in vivo

post-pmcao injections with vpa could decrease the brain infarct volume. postinsult treatment with vpa also reduced the number of microglia, suppressed microglial activation, and inhibited other inflammatory markers in the ischemic brain. the reduction in acetylated histone h3 was prevented by treatment with vpa. moreover, vpa superinduced heat-shock protein 70 and blocked pmcao-induced down-regulation of cyclooxygenase-2. the sensory, motor, and reflex performance of pmcao rats was improved by vpa treatment [1].

Drug interactions

Potentially hazardous interactions with other drugs
Antibacterials: metabolism possibly inhibited by erythromycin; avoid with pivmecillinam; concentration reduced by carbapenems - avoid.
Antidepressants: antagonise anticonvulsant effect; avoid with St John’s wort.
Antiepileptics: concentration reduced by carbamazepine; concentration of active carbamazepine metabolite increased; increased concentration of lamotrigine, phenobarbital, rufinamide and possibly ethosuximide; sometimes reduces concentration of active metabolite of oxcarbazepine; alters phenytoin concentration; phenytoin and phenobarbital reduce valproate concentration; hyperammonaemia and CNS toxicity with topiramate.
Antimalarials: mefloquine antagonises anticonvulsant effect.
Antipsychotics: antagonise anticonvulsant effect; increased neutropenia with olanzapine; possibly increases or decreases concentration of clozapine; possibly increases quetiapine concentration.
Ciclosporin: variable ciclosporin blood level response.
Orlistat: possibly increased risk of convulsions.
Sodium oxybate: concentration of sodium oxybate increased.
Ulcer-healing drugs: metabolism inhibited by cimetidine, increased concentration.

Metabolism

Valproic acid is extensively metabolised in the liver, a large part by glucuronidation (up to 60%) and the rest by a variety of complex pathways (up to 45%).
It is excreted in the urine almost entirely in the form of its metabolites; small amounts are excreted in faeces and expired air.

storage

room temperature (desiccate)

References

1) Phiel et al. (2001), Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen; J. Biol. Chem. 276 36734 2) Kim et al. (2007), Histone deacetylase inhibitors exhibit anti-inflammatory and neuroprotective effects in a rat permanent ischemic model of stroke: multiple mechanisms of action; J. Pharmacol. Exp. Ther. 321 892 3) Qing et al. (2008), Valproic acid inhibits Abeta production, neuritic plaque formation, and behavioral deficits in Alzheimer’s mouse models; J. Exp. Med. 205 2781 4) Hangfu et al. (2008), Induction of pluripotent stem cells by defined factors is greatly improved by small molecule compounds; Nat. Biotechnol. 26 795