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HomeProduct name listO-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

  • CAS NO.:132489-69-1
  • Empirical Formula: C15H19N3O7
  • Molecular Weight: 353.33
  • MDL number: MFCD00145022
  • EINECS: 1533716-785-6
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-11-06 15:28:16
O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI Structural

What is O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI?

Description

Proteins can be modified post-translationally by the addition of O-linked N-acetylglucosamine (O-GlcNAc). Nuclear cytoplasmic O-GlcNAcase and acetyltransferase (NCOAT) is a β-N-acetylglucosaminidase that removes GlcNAc from O-glycosylated proteins. PUGNAc is a (phenylcarbamoyl)oxime analog of GlcNAc that reversibly inhibits NCOAT (Ki = 40-110 nM). It also less potently inhibits other hexosaminidases and exochitinases. (Z)-PUGNAc is a stereoisomer of PUGNAc that is a more potent inhibitor of NCOAT than the (E) isomer, both in vitro and in cells.

Chemical properties

White to Off-White Solid

The Uses of O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

O-(2-acetamido-2deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate (PUGNAc) has been used to evaluate the effects of silibinin on O-GlcNAc levels of glycoproteins in Adult Retinal Pigment Epithelial-19 (ARPE-19) cells. It has also been used as a component of the HEPES lysis buffer for rat brain samples.

The Uses of O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

An inhibitor of O-GlcNAcase, hexosaminidase A, and hexosaminidase B

What are the applications of Application

(Z)-Pugnac is an O-GlcNAcase inhibitor that undergoes Beckmann rearrangement to promote insulin resistance in adipocytes.

Biological Activity

O -GlcNAc- β - N -acetylglucosaminidase ( O -GlcNAcase) and β -hexosaminidase inhibitor (K i values are 46 and 36 nM respectively) that increases O -GlcNAc levels ~ 2-fold in HT29 cells. Z -linked isomer is more potent than the E isomer.

Biochem/physiol Actions

O-(2-acetamido-2deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate (PUGNAc) induces insulin resistance in 3T3-L1 adipocytes by reducing insulin-prompting phosphorylation of protein kinase B (Akt) and glycogen synthase kinase 3β (GSK3β).

storage

Desiccate at -20°C

References

1) Macauley et al. (2005), O-GlcNAcase uses substrate-assisted catalysis: kinetic analysis and development of highly selective mechanism-inspired inhibitors; J. Biol. Chem., 280 25313 2) Kneass and Marchase (2005), Protein O-GlcNAc modulates motility-associated signaling intermediates in neutrophils; J. Biol. Chem., 280 14579 3) Zou et al. (2007), The protective effects of PUGNAC on cardiac function after trauma-hemorrhage are mediated via increased protein O-GlcNAc levels; Shock, 27 402 4) Arias et al. (2004), Prolonged incubation in PUGNAc results in increased protein O-Linked glycosylation and insulin resistance in rat skeletal muscle; Diabetes, 53 921

Properties of O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

Melting point: 172-175°C
Density  1.53
storage temp.  -20°C
solubility  Soluble in DMSO (up to 35 mg/ml)
form  solid
pka 11.85±0.70(Predicted)
color  White
BRN  4274031
Stability: Moisture and Temperature Sensitive

Safety information for O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P280:Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352:IF ON SKIN: wash with plenty of soap and water.

Computed Descriptors for O-[2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYLI

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