3-METHYLADENINE
Synonym(s):3-MA;3-Methyladenine;6-Amino-3-methylpurine;Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem
- CAS NO.:5142-23-4
- Empirical Formula: C6H7N5
- Molecular Weight: 149.15
- MDL number: MFCD00010531
- EINECS: 225-908-6
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 20:33:22
What is 3-METHYLADENINE?
Description
Autophagy is a multi-
Chemical properties
White powder
The Uses of 3-METHYLADENINE
3-Methyladenine (3-MA) inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.
The Uses of 3-METHYLADENINE
A multi-use inhibitor that protects cerebellar granule cells from apoptosis.
The Uses of 3-METHYLADENINE
A synthetic intermediate and a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis post serum/potassium deprivation
What are the applications of Application
Autophagy Inhibitor, 3-MA is a multi-use inhibitor that inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases.
Definition
ChEBI: A methyladenine that is adenine substituted with a methyl group at position N-3.
General Description
A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
Biochem/physiol Actions
3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).
storage
+4°C
References
1) Seglen and Gordon?et al.?(1982),?3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes; Proc. Natl. Acad. Sci. USA,?79?1889 2) Blommaart?et al. (1997),?The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes; Eur. J. Biochem.,?243?240 3) McFarland?et al. (2012),?Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity; Arch. Toxicol.,?86?275 4) Zhao?et al. (2014),?Inhibition of autophagy strengthens celastrol-induced apoptosis in human pancreatic cancer in vitro and in vivo models; Curr. Mol. Med.,?14?555
Properties of 3-METHYLADENINE
Melting point: | ~300 °C (dec.)(lit.) |
Boiling point: | 240℃ |
Density | 1.60 |
refractive index | 1.8070 (estimate) |
Flash point: | 99℃ |
storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
solubility | Soluble in DMSO (up to 3 mg/ml), or in DMF (up to 10 mg/ml). |
pka | 6.77±0.20(Predicted) |
form | Powder |
color | White |
BRN | 146087 |
Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20° for up to 3 months. |
Safety information for 3-METHYLADENINE
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention. P403:Store in a well-ventilated place. |
Computed Descriptors for 3-METHYLADENINE
New Products
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