Contact us: +91 9550333722 040 - 40102781
Structured search
India
Choose your country
Different countries will display different contents
Try our best to find the right business for you.
My chemicalbook

Welcome back!

HomeProduct name listMES monohydrate

MES monohydrate

Synonym(s):MES;2-(N-Morpholino)ethanesulfonic acid;4-Morpholineethanesulfonic acid;2-(N-Morpholino)ethanesulfonic acid hydrate;2-Morpholinoethanesulfonic acid monohydrate

  • CAS NO.:145224-94-8
  • Empirical Formula: C6H15NO5S
  • Molecular Weight: 213.25
  • MDL number: MFCD00149409
  • EINECS: 224-632-3
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-25 16:21:11
MES monohydrate Structural

What is MES monohydrate?

Description

MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.

Chemical properties

white crystalline powder

The Uses of MES monohydrate

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.

The Uses of MES monohydrate

MES monohydrate is a biological buffer often referred to as Good?s buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.

The Uses of MES monohydrate

MES Monohydrate has been used:

  • To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
  • As a buffering agent to stabilize enzymatic solutions
  • As a component of PAGE running buffer

What are the applications of Application

MES monohydrate is often used as a running buffer for resolving very small proteins

What are the applications of Application

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
MES Monohydrate has been used:
To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.
As a buffering agent to stabilize enzymatic solutions.
As a component of PAGE running buffer.

General Description

buffer substance MES

Preparation and handling

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Precautions

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

Properties of MES monohydrate

Melting point: >300 °C(lit.)
storage temp.  Sealed in dry,Room Temperature
solubility  H2O: 0.5 M at 20 °C, clear
form  Powder, Crystals or Flakes
color  White to pale yellow and/or orange
Odor Odorless
PH 2.5-4.0 (25℃, 0.5M in H2O)
pka 6.1(at 25℃)
PH Range 5.5 - 6.7
Water Solubility  Soluble in water.
λmax λ: 260 nm Amax: 0.025
λ: 280 nm Amax: 0.020
Merck  14,5902
BRN  141319
InChI InChI=1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
CAS DataBase Reference 145224-94-8(CAS DataBase Reference)

Safety information for MES monohydrate

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P264:Wash hands thoroughly after handling.
P264:Wash skin thouroughly after handling.
P321:Specific treatment (see … on this label).
P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P332+P313:IF SKIN irritation occurs: Get medical advice/attention.
P337+P313:IF eye irritation persists: Get medical advice/attention.
P405:Store locked up.

Computed Descriptors for MES monohydrate

InChIKey MIIIXQJBDGSIKL-UHFFFAOYSA-N
SMILES S(=O)(=O)(O)CCN1CCOCC1.O

Related products of tetrahydrofuran

You may like

  • 145224-94-8 2-morpholinoethanesulfonic acid 98%
    145224-94-8 2-morpholinoethanesulfonic acid 98%
    145224-94-8
    View Details
  • 2-(N-Morpholino)Ethane-Sulfonic Acid CAS 145224-94-8
    2-(N-Morpholino)Ethane-Sulfonic Acid CAS 145224-94-8
    145224-94-8
    View Details
  • MES buffer soln. CAS 145224-94-8
    MES buffer soln. CAS 145224-94-8
    145224-94-8
    View Details
  • MES Monohydrate Buffer for tissue culture CAS 145224-94-8
    MES Monohydrate Buffer for tissue culture CAS 145224-94-8
    145224-94-8
    View Details
  • MES Monohydrate Buffer for cell culture CAS 145224-94-8
    MES Monohydrate Buffer for cell culture CAS 145224-94-8
    145224-94-8
    View Details
  • Morpholino ethane sulphonic acid, monohydrate CAS 145224-94-8
    Morpholino ethane sulphonic acid, monohydrate CAS 145224-94-8
    145224-94-8
    View Details
  • Mes monohydrate 95% CAS 145224-94-8
    Mes monohydrate 95% CAS 145224-94-8
    145224-94-8
    View Details
  • MORPHOLINOETHANE SULPHONIC ACID MONOHYDRATE Extra Pure CAS 145224-94-8
    MORPHOLINOETHANE SULPHONIC ACID MONOHYDRATE Extra Pure CAS 145224-94-8
    145224-94-8
    View Details
Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.