4-Morpholineethanesulfonic acid

Synonym(s):2-(N-Morpholino) ethanesulfonic acid;2-(N-Morpholino)ethanesulfonic Acid;4-Morpholineethanesulfonic acid

CAS NO.：4432-31-9
Empirical Formula: C6H13NO4S
Molecular Weight: 195.24
MDL number: MFCD00006181
EINECS: 224-632-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-08-24 19:19:26

4-Morpholineethanesulfonic acid Structural

What is 4-Morpholineethanesulfonic acid?

Chemical properties

White/clear crystalline powder

The Uses of 4-Morpholineethanesulfonic acid

Biological buffer.

The Uses of 4-Morpholineethanesulfonic acid

MES solution has been used to activate microspheres. It has also been used as a component of activating solution to activate carboxyl groups of poly lactic-co-glycolic acid (PLGA).

Definition

ChEBI: 2-(N-morpholino)ethanesulfonic acid is a Good's buffer substance, pKa = 6.15 at 20 ℃. It is an organosulfonic acid and a MES. It is a conjugate acid of a 2-(N-morpholino)ethanesulfonate. It is a tautomer of a 2-(N-morpholiniumyl)ethanesulfonate.

What are the applications of Application

MES solution is used as a running buffer for resolving very small proteins

What are the applications of Application

MES is a zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays

General Description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Flammability and Explosibility

Not classified

storage

Store at RT

Purification Methods

Crystallise MES from hot EtOH containing a little water. The picrate crystallises from EtOH and has m 178.8-182o. [Malkiel & Mason J Org Chem 8 199 1943, Beilstein 27 III/IV 370.]

Properties of 4-Morpholineethanesulfonic acid

Melting point:	>300 °C(lit.)
Boiling point:	102°C
Density	1.208 (estimate)
refractive index	1.5500 (estimate)
storage temp.	2-8°C
solubility	H₂O: 0.5 M at 20 °C, clear
pka	pKa at 0.1M ionic: 0°, 6.38; 20°, 6.15; 37°, 5.98; pKa1 1.99; pKa2 6.21; pKa/°C -0.011
form	Powder
color	White
PH	2.5-4.0 (25℃ in neat)
PH Range	5.5 - 6.7
Water Solubility	185.2g/L at 20℃
λmax	λ: 260 nm Amax: 0.01 λ: 280 nm Amax: 0.01
Merck	13,5929
BRN	6350956
Stability:	Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference	4432-31-9(CAS DataBase Reference)
EPA Substance Registry System	4-Morpholineethanesulfonic acid (4432-31-9)

Safety information for 4-Morpholineethanesulfonic acid

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection.