HEPES

Synonym(s):HEPES;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid;HEPES solution;N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);N-2-Hydroxyethylpiperazine-N?-2-ethanesulfonic Acid

CAS NO.：7365-45-9
Empirical Formula: C8H18N2O4S
Molecular Weight: 238.3
MDL number: MFCD00006158
EINECS: 230-907-9
SAFETY DATA SHEET (SDS)
Update Date: 2024-05-10 14:01:29

What is HEPES?

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

The Uses of HEPES

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

What are the applications of Application

HEPES Buffer Solution (1M) is a zwitterionic, all-purpose "Good′s" buffer for use in biological research

What are the applications of Application

HEPES is an N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5.

Properties of HEPES

Melting point:	234-238 °C
Boiling point:	408℃[at 101 325 Pa]
Density	1.07 g/mL at 20 °C
storage temp.	2-8°C
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	powder
color	White
Water Solubility	Soluble

Safety information for HEPES

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection.