Contact us: +91 9550333722 040 - 40102781
Structured search
India
Choose your country
Different countries will display different contents
Try our best to find the right business for you.
My chemicalbook

Welcome back!

HomeProduct name listDL-Dithiothreitol

DL-Dithiothreitol

Synonym(s):DTT;1,4-dithiothreitol;cleland′s reagent;Cleland’s reagent;dithiothreitol, 1,4-

  • CAS NO.:27565-41-9
  • Empirical Formula: C4H10O2S2
  • Molecular Weight: 154.25
  • MDL number: MFCD00004877
  • EINECS: 248-531-9
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-12-18 14:15:30
DL-Dithiothreitol Structural

What is DL-Dithiothreitol?

Chemical properties

White solid

The Uses of DL-Dithiothreitol

Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins.

The Uses of DL-Dithiothreitol

DTT is a cell permeable protective agent for Thiol groups.

What are the applications of Application

DTT is a cell permeable protective agent for Thiol groups which keeps monothiols in the reduced state and reduces disulfides

Preparation

The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis.

Definition

ChEBI: D-1,4-dithiothreitol is a 1,4-dithiothreitol. It is an enantiomer of a L-1,4-dithiothreitol.

Purification Methods

Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.]

Properties of DL-Dithiothreitol

Melting point: 41-44 °C(lit.)
Boiling point: 125-130 °C (2 mmHg)
Density  1.04 g/mL at 20 °C
refractive index  1.5200 (estimate)
Flash point: >230 °F
storage temp.  2-8°C
solubility  H2O: 50 mg/mL, clear, colorless
form  Liquid
color  White
Water Solubility  Soluble
Merck  3376
CAS DataBase Reference 27565-41-9(CAS DataBase Reference)
EPA Substance Registry System 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-(27565-41-9)

Safety information for DL-Dithiothreitol

Computed Descriptors for DL-Dithiothreitol

Related products of tetrahydrofuran

You may like

Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.