DL-Dithiothreitol
Synonym(s):DTT;1,4-dithiothreitol;cleland′s reagent;Cleland’s reagent;dithiothreitol, 1,4-
- CAS NO.:27565-41-9
- Empirical Formula: C4H10O2S2
- Molecular Weight: 154.25
- MDL number: MFCD00004877
- EINECS: 248-531-9
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-03-26 17:16:27
What is DL-Dithiothreitol?
Chemical properties
White solid
The Uses of DL-Dithiothreitol
Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins.
The Uses of DL-Dithiothreitol
DTT is a cell permeable protective agent for Thiol groups.
What are the applications of Application
DTT is a cell permeable protective agent for Thiol groups which keeps monothiols in the reduced state and reduces disulfides
Preparation
The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis.
Definition
ChEBI: D-1,4-dithiothreitol is a 1,4-dithiothreitol. It is an enantiomer of a L-1,4-dithiothreitol.
Purification Methods
Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.]
Properties of DL-Dithiothreitol
Melting point: | 41-44 °C(lit.) |
Boiling point: | 125-130 °C (2 mmHg) |
Density | 1.04 g/mL at 20 °C |
refractive index | 1.5200 (estimate) |
Flash point: | >230 °F |
storage temp. | 2-8°C |
solubility | H2O: 50 mg/mL, clear, colorless |
form | Liquid |
color | White |
Water Solubility | Soluble |
Merck | 3376 |
CAS DataBase Reference | 27565-41-9(CAS DataBase Reference) |
EPA Substance Registry System | 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-(27565-41-9) |
Safety information for DL-Dithiothreitol
Computed Descriptors for DL-Dithiothreitol
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