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HomeProduct name list5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid)

Synonym(s):DTNB;Ellman’s Reagent;DTNB, Ellman’s Reagent;3-Carboxy-4-nitrophenyl disulfide;5,5?-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem

  • CAS NO.:69-78-3
  • Empirical Formula: C14H8N2O8S2
  • Molecular Weight: 396.35
  • MDL number: MFCD00007140
  • EINECS: 200-714-4
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-11-19 23:02:33
5,5′-Dithiobis(2-nitrobenzoic acid) Structural

What is 5,5′-Dithiobis(2-nitrobenzoic acid)?

Chemical properties

Crystalline

The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.

The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

What are the applications of Application

5,5′-Dithio-bis-(2-nitrobenzoic Acid) is a sulfhydryl reagent used to characterize reactive thiol groups

Definition

ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.

General Description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Properties of 5,5′-Dithiobis(2-nitrobenzoic acid)

Melting point: 240-245 °C (dec.)(lit.)
Boiling point: 671.9±55.0 °C(Predicted)
Density  1.67
refractive index  1.6900 (estimate)
storage temp.  Keep in dark place,Sealed in dry,Room Temperature
solubility  Do you have solubility information on this product that you would like to share
pka 1.57±0.25(Predicted)
form  Yellow solid
color  Pale Yellow to Light Yellow
Water Solubility  Soluble in water and ethanol.
Merck  14,3551
BRN  1896821
Stability: Stable. Incompatible with strong bases, strong oxidizing agents.
CAS DataBase Reference 69-78-3(CAS DataBase Reference)
EPA Substance Registry System Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)

Safety information for 5,5′-Dithiobis(2-nitrobenzoic acid)

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P264:Wash hands thoroughly after handling.
P264:Wash skin thouroughly after handling.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405:Store locked up.

Computed Descriptors for 5,5′-Dithiobis(2-nitrobenzoic acid)

InChIKey KIUMMUBSPKGMOY-UHFFFAOYSA-N

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