5,5′-Dithiobis(2-nitrobenzoic acid)
Synonym(s):DTNB;Ellman’s Reagent;DTNB, Ellman’s Reagent;3-Carboxy-4-nitrophenyl disulfide;5,5?-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem
- CAS NO.:69-78-3
- Empirical Formula: C14H8N2O8S2
- Molecular Weight: 396.35
- MDL number: MFCD00007140
- EINECS: 200-714-4
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-12-18 14:07:02
What is 5,5′-Dithiobis(2-nitrobenzoic acid)?
Chemical properties
Crystalline
The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)
5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.
The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)
5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.
The Uses of 5,5′-Dithiobis(2-nitrobenzoic acid)
Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
What are the applications of Application
5,5′-Dithio-bis-(2-nitrobenzoic Acid) is a sulfhydryl reagent used to characterize reactive thiol groups
Definition
ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.
General Description
5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.
Properties of 5,5′-Dithiobis(2-nitrobenzoic acid)
Melting point: | 240-245 °C (dec.)(lit.) |
Boiling point: | 671.9±55.0 °C(Predicted) |
Density | 1.67 |
refractive index | 1.6900 (estimate) |
storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
solubility | Do you have solubility information on this product that you would like to share |
pka | 1.57±0.25(Predicted) |
form | Yellow solid |
color | Pale Yellow to Light Yellow |
Water Solubility | Soluble in water and ethanol. |
Merck | 14,3551 |
BRN | 1896821 |
Stability: | Stable. Incompatible with strong bases, strong oxidizing agents. |
CAS DataBase Reference | 69-78-3(CAS DataBase Reference) |
EPA Substance Registry System | Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3) |
Safety information for 5,5′-Dithiobis(2-nitrobenzoic acid)
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up. |
Computed Descriptors for 5,5′-Dithiobis(2-nitrobenzoic acid)
InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N |
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