3,3',5,5'-Tetramethylbenzidine dihydrochloride

Synonym(s):4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

3,3',5,5'-Tetramethylbenzidine dihydrochloride is white powder

3,3',5,5'-Tetramethylbenzidine dihydrochloride is a peroxidase substrate suitable for use in ELISA procedures.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

3,3′,5,5′-Tetramethylbenzidine dihydrochloride anhydrous is a peroxidase substrate suitable for use in ELISA procedures

Melting point:	≥300 °C
Flash point:	54 °C
storage temp.	2-8°C
form	tablet
color	White to off-white
Sensitive	Moisture & Light Sensitive
InChI	InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;/h5-8H,17-18H2,1-4H3;1H
CAS DataBase Reference	64285-73-0(CAS DataBase Reference)
EPA Substance Registry System	[1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-, dihydrochloride (64285-73-0)

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up.