Contact us: +91 9550333722 040 - 40102781
Structured search
India
Choose your country
Different countries will display different contents
Try our best to find the right business for you.
My chemicalbook

Welcome back!

HomeProduct name list3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

  • CAS NO.:207738-08-7
  • Empirical Formula: C16H23ClN2O
  • Molecular Weight: 294.82
  • MDL number: MFCD00150104
  • EINECS: 688-511-0
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2023-06-08 09:03:06
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Structural

What is 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE?

Description

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

Chemical properties

Off-white fluffy powder

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Convenient water-soluble form of TMB for determination of peroxidase.

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

What are the applications of Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate is a chromophore substrate in ELISA assays

General Description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Properties of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Melting point: >300°C
storage temp.  2-8°C
solubility  Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
form  Powder
Sensitive  Moisture & Light Sensitive
CAS DataBase Reference 207738-08-7

Safety information for 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
ghs
Health Hazard
GHS08
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H341:Germ cell mutagenicity
Precautionary Statement Codes P201:Obtain special instructions before use.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405:Store locked up.

Computed Descriptors for 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

InChIKey NYNRGZULARUZCC-UHFFFAOYSA-N

Related products of tetrahydrofuran

You may like

  • 3,3,5,5-Tetramethyl Benzidine Dihydrochloride Hydrate (TMB.2HCl.xH2O) extrapure CAS 207738-08-7
    3,3,5,5-Tetramethyl Benzidine Dihydrochloride Hydrate (TMB.2HCl.xH2O) extrapure CAS 207738-08-7
    207738-08-7
    View Details
  • 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate CAS 207738-08-7
    3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate CAS 207738-08-7
    207738-08-7
    View Details
  • 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate CAS 207738-08-7
    3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate CAS 207738-08-7
    207738-08-7
    View Details
  • Atracurium Besylate EP Impurity C
    Atracurium Besylate EP Impurity C
    86293-35-8
    View Details
  • Atracurium Besylate EP Impurity D
    Atracurium Besylate EP Impurity D
    87046-72-8
    View Details
  • Azithromycin EP Impurity C
    Azithromycin EP Impurity C
    620169-47-3
    View Details
  • Azithromycin Imp D
    Azithromycin Imp D
    612069-26-8
    View Details
  • Ceftaroline fosamil Acetate Hydrate
    Ceftaroline fosamil Acetate Hydrate
    400827-55-6
    View Details
Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.