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HomeProduct name list3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

  • CAS NO.:207738-08-7
  • Empirical Formula: C16H23ClN2O
  • Molecular Weight: 294.82
  • MDL number: MFCD00150104
  • EINECS: 688-511-0
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2023-06-08 09:03:06
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Structural

What is 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE?

Description

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

Chemical properties

Off-white fluffy powder

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Convenient water-soluble form of TMB for determination of peroxidase.

The Uses of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

What are the applications of Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate is a chromophore substrate in ELISA assays

General Description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Properties of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Melting point: >300°C
storage temp.  2-8°C
solubility  Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
form  Powder
Sensitive  Moisture & Light Sensitive
CAS DataBase Reference 207738-08-7

Safety information for 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
ghs
Health Hazard
GHS08
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H341:Germ cell mutagenicity
Precautionary Statement Codes P201:Obtain special instructions before use.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405:Store locked up.

Computed Descriptors for 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

InChIKey NYNRGZULARUZCC-UHFFFAOYSA-N

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