MOPS

Chemical properties

White/clear crystalline powder

The Uses of MOPS

3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.
MOPS has been used as:
a cell culture additive component in lentiviral particle production.
as a buffering agent in microbial growth medium and nuclei extraction buffer.
as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum.
as a buffer in capillary-zone electrophoresis to test performance.
for the dilution of proteins from algal samples.

The Uses of MOPS

MOPS acts as a multi-purpose buffering agent used in various biological research.

What are the applications of Application

MOPS solution is a morpholino propanesulfonic acid buffer

What are the applications of Application

MOPS, Free Acid is an MES structural analog and buffer agent with a pKa close to physiological pH.

Definition

ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.

General Description

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Flammability and Explosibility

Not classified

Synthesis

Morpholine and 1,3-Propanesultone are added to absolute ethanol for reaction. The crude product synthesized is purified to obtain 3-Morpholinopropanesulfonic acid.