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HomeProduct name listBIS-Tris

BIS-Tris

Synonym(s):[ bis(2-Hydroxyethyl)imino]- tris(hydroxymethyl)methane;[bis(2-Hydroxyethyl)imino]-tris(hydroxymethyl)methane;2,2-Bis(hydroxyethyl)-(iminotris)- (hydroxymethyl)-methane;2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol;2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol

  • CAS NO.:6976-37-0
  • Empirical Formula: C8H19NO5
  • Molecular Weight: 209.24
  • MDL number: MFCD00002853
  • EINECS: 230-237-7
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-25 16:21:11
BIS-Tris Structural

What is BIS-Tris?

Chemical properties

White/clear crystalline powder

The Uses of BIS-Tris

It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.

The Uses of BIS-Tris

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

The Uses of BIS-Tris

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

What are the applications of Application

BIS-Tris is an inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant

Definition

ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

Properties of BIS-Tris

Melting point: 104 °C(lit.)
Boiling point: 466.2±40.0 °C(Predicted)
Density  1.346±0.06 g/cm3(Predicted)
vapor pressure  0Pa at 25℃
storage temp.  Inert atmosphere,Room Temperature
solubility  H2O: 1 M at 20 °C, clear, colorless
form  Solution
color  Yellow to orange
Odor Odorless
PH 9.0-10.1 (20-25℃, 1M in water)
pka 6.5(at 25℃)
PH Range 5.8 - 7.2
Water Solubility  soluble
Sensitive  Air Sensitive
λmax λ: 280 nm Amax: 0.15
BRN  2205275
CAS DataBase Reference 6976-37-0(CAS DataBase Reference)
EPA Substance Registry System 1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)

Safety information for BIS-Tris

Signal word Danger
Pictogram(s)
ghs
Corrosion
Corrosives
GHS05
GHS Hazard Statements H318:Serious eye damage/eye irritation
Precautionary Statement Codes P280:Wear protective gloves/protective clothing/eye protection/face protection.

Computed Descriptors for BIS-Tris

InChIKey OWMVSZAMULFTJU-UHFFFAOYSA-N

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