BIS-Tris
Synonym(s):[ bis(2-Hydroxyethyl)imino]- tris(hydroxymethyl)methane;[bis(2-Hydroxyethyl)imino]-tris(hydroxymethyl)methane;2,2-Bis(hydroxyethyl)-(iminotris)- (hydroxymethyl)-methane;2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol;2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
- CAS NO.:6976-37-0
- Empirical Formula: C8H19NO5
- Molecular Weight: 209.24
- MDL number: MFCD00002853
- EINECS: 230-237-7
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-25 16:21:11
What is BIS-Tris?
Chemical properties
White/clear crystalline powder
The Uses of BIS-Tris
It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.
The Uses of BIS-Tris
Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.
Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.
The Uses of BIS-Tris
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
What are the applications of Application
BIS-Tris is an inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant
Definition
ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.
Flammability and Explosibility
Not classified
Purification Methods
Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.
Properties of BIS-Tris
Melting point: | 104 °C(lit.) |
Boiling point: | 466.2±40.0 °C(Predicted) |
Density | 1.346±0.06 g/cm3(Predicted) |
vapor pressure | 0Pa at 25℃ |
storage temp. | Inert atmosphere,Room Temperature |
solubility | H2O: 1 M at 20 °C, clear, colorless |
form | Solution |
color | Yellow to orange |
Odor | Odorless |
PH | 9.0-10.1 (20-25℃, 1M in water) |
pka | 6.5(at 25℃) |
PH Range | 5.8 - 7.2 |
Water Solubility | soluble |
Sensitive | Air Sensitive |
λmax | λ: 280 nm Amax: 0.15 |
BRN | 2205275 |
CAS DataBase Reference | 6976-37-0(CAS DataBase Reference) |
EPA Substance Registry System | 1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0) |
Safety information for BIS-Tris
Signal word | Danger |
Pictogram(s) |
Corrosion Corrosives GHS05 |
GHS Hazard Statements |
H318:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for BIS-Tris
InChIKey | OWMVSZAMULFTJU-UHFFFAOYSA-N |
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