Contact us: +91 9550333722 040 - 40102781
Structured search
India
Choose your country
Different countries will display different contents
Try our best to find the right business for you.
My chemicalbook

Welcome back!

HomeProduct name listTricine

Tricine

Synonym(s):Tricine;Tricine, N-Tris(hydroxymethyl)methylglycine;Tris Buffer substance;Tris(hydroxymethyl)aminomethane Buffer substance;Tris(hydroxymethyl)aminomethane hydrochloride buffer solution

  • CAS NO.:5704-04-1
  • Empirical Formula: C6H13NO5
  • Molecular Weight: 179.17
  • MDL number: MFCD00004277
  • EINECS: 227-193-6
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-25 16:21:11
Tricine Structural

What is Tricine?

Chemical properties

White/clear crystalline powder

The Uses of Tricine

Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets.
A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.
A zwitterionic amino acid commonly useful in the pH range of 7.4-8.8. Has a pKa 8.05 at 25°C.

What are the applications of Application

Tricine is a buffer component for separation of low molecular weight peptides

Definition

ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.

General Description

Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.

Biological Activity

Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.

Purification Methods

Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

Properties of Tricine

Melting point: 186-188 °C (dec.)
Boiling point: 311.69°C (rough estimate)
Density  1.05 g/mL at 20 °C
vapor pressure  0.002-0.004Pa at 20-25℃
refractive index  1.4240 (estimate)
storage temp.  Keep in dark place,Inert atmosphere,Room temperature
solubility  H2O: 4 M at 20 °C, clear, colorless
form  crystalline
color  clear colorless (40 % (w/w) in H2O) solution
Odor Odorless
PH 4.0-6.0 (1M in H2O)
pka 8.1(at 25℃)
PH Range 7.4 - 8.8
Water Solubility  soluble
Merck  14,9651
BRN  1937804
Stability: Stable.
CAS DataBase Reference 5704-04-1(CAS DataBase Reference)
EPA Substance Registry System Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)

Safety information for Tricine

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H320:Serious eye damage/eye irritation
Precautionary Statement Codes P403:Store in a well-ventilated place.

Computed Descriptors for Tricine

Related products of tetrahydrofuran

You may like

Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.