1-Methoxy-5-methylphenazinium methyl sulfate
Synonym(s):1-Methoxyphenazine methosulfate
- CAS NO.：65162-13-2
- Empirical Formula: C15H16N2O5S
- Molecular Weight: 336.36
- MDL number: MFCD00040648
- EINECS: 265-579-6
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 20:33:22
What is 1-Methoxy-5-methylphenazinium methyl sulfate?
The Uses of 1-Methoxy-5-methylphenazinium methyl sulfate
1-Methoxy-5-methylphenazinium methyl sulfate (PMS) has a redox potential of +63 mV. PMS is often used as an electron carrier for NADH tetrazolium and is a useful reagent for NAD(P)H-tetrazolium-based analytical systems. It is mainly used in the cytotoxic proliferation reagent CCK-8 experiment.
What are the applications of Application
1-Methoxy-5-methylphenazinium methyl sulfate is a photochemically stable mediator of electron transport that works on various electron acceptors including tetrazolium dyes.
Mechanism of action
1-Methoxy-5-methylphenazinium methyl sulfate contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazolyl monosodium salt], which is reduced to a highly water-soluble yellow formazan dye by dehydrogenase in cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate. The amount of formazan generated is proportional to the number of living cells. Therefore, this property can directly analyze cell proliferation and toxicity. The main uses of this reagent are drug screening, cell proliferation assay, cytotoxicity assay, and tumour drug sensitivity test.
Properties of 1-Methoxy-5-methylphenazinium methyl sulfate
| Melting point: | 172 °C |
| storage temp. | Inert atmosphere,Room Temperature |
| solubility | Water: 1 mg/ml |
| form | powder to crystal |
| color | Orange to Brown to Dark purple |
| InChI | InChI=1S/C14H13N2O.CH4O4S/c1-16-11-7-4-3-6-10(11)15-14-12(16)8-5-9-13(14)17-2;1-5-6(2,3)4/h3-9H,1-2H3;1H3,(H,2,3,4)/q+1;/p-1 |
| CAS DataBase Reference | 65162-13-2(CAS DataBase Reference) |
Safety information for 1-Methoxy-5-methylphenazinium methyl sulfate
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07  Health Hazard GHS08 |
| GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H341:Germ cell mutagenicity H351:Carcinogenicity H373:Specific target organ toxicity, repeated exposure |
| Precautionary Statement Codes |
P202:Do not handle until all safety precautions have been read and understood. P260:Do not breathe dust/fume/gas/mist/vapours/spray. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P308+P313:IF exposed or concerned: Get medical advice/attention. |
Computed Descriptors for 1-Methoxy-5-methylphenazinium methyl sulfate
| InChIKey | MASUWVVNWALEEM-UHFFFAOYSA-M |
| SMILES | S([O-])(=O)(=O)OC.O(C1=CC=CC2=[N+](C3=CC=CC=C3N=C12)C)C |
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