XTT sodium salt

Synonym(s):2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt;2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt

CAS NO.：111072-31-2
Empirical Formula: C22H18N7NaO13S2
Molecular Weight: 675.53
MDL number: MFCD00191206
EINECS: 601-039-1
SAFETY DATA SHEET (SDS)
Update Date: 2023-04-23 13:52:06

What is XTT sodium salt?

The Uses of XTT sodium salt

Tetrazolium derivative useful in cancer research.XTT sodium salt is used in conjunction with phenazine methosulfate to screen human cancer cell lines. It is also used to study fungal cell damage. It is involved in testing antimicrobial susceptibility of staphylococci and in candida biofilm research. Further, it is used in an assay for cell viability and cell proliferation.

The Uses of XTT sodium salt

Useful in AIDS and cancer testing.

What are the applications of Application

XTT sodium salt is used in an assay for cell viability and cell proliferation

General Description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Biochem/physiol Actions

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Properties of XTT sodium salt

Melting point:	285 ℃ (decompose)
storage temp.	2-8°C
solubility	hot water: 2.5 mg/mL
form	powder
color	Yellow
Water Solubility	Soluble in hot water at 2.5mg/ml.Soluble in water, dimethyl sulfoxide, dimethyl formamide and phosphate buffered saline.at pH =7.2.
λmax	286 nm
Biological Applications	Cell proliferation assays; cytotoxicity assays; ecotoxicity assays; neurotoxicity assays; antimicrobial susceptibility testing assays; antifungal susceptibility testing assays; cardiac valve viability assays; cell viability assay
CAS DataBase Reference	111072-31-2(CAS DataBase Reference)

Safety information for XTT sodium salt

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection.