2,3,5-Triphenyltetrazolium chloride
Synonym(s):TPTZ;TTZ;2,3,5-Triphenyltetrazolium chloride;TTC solution;TTC supplement
- CAS NO.：298-96-4
- Empirical Formula: C19H15ClN4
- Molecular Weight: 334.8
- MDL number: MFCD00011963
- EINECS: 206-071-6
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 23:02:33
What is 2,3,5-Triphenyltetrazolium chloride?
Chemical properties
white powder
The Uses of 2,3,5-Triphenyltetrazolium chloride
2,3,5-Triphenyltetrazolium chloride is a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia
The Uses of 2,3,5-Triphenyltetrazolium chloride
2,3,5-Triphenyl-2H-tetrazolium chloride is used as a dye in cell biological studies.
The Uses of 2,3,5-Triphenyltetrazolium chloride
In analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between a-ketols and simple aldehydes. For staining plant and animal tissue. Germination indicator in testing the ability of seeds to germinate: G. Lakon, Ber. Dtsch. Bot. Ges. 60, 299, 434 (1942). In histochemical studies. In determination of antibiotics; of dehydrogenases.
Definition
ChEBI: An organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion.
General Description
2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.
Purification Methods
Crystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.]
Properties of 2,3,5-Triphenyltetrazolium chloride
| Melting point: | 250 °C(lit.) |
| Boiling point: | 490.96°C (rough estimate) |
| Density | 1.1704 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | 2-8°C |
| solubility | H2O: may be clear to slightly hazy |
| form | powder |
| color | faint yellow |
| Water Solubility | Soluble in water, ethanol and acetone. Insoluble in ether. |
| λmax | 247 nm |
| Sensitive | Light Sensitive & Hygroscopic |
| Merck | 14,9744 |
| BRN | 3923356 |
| Stability: | Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents. |
| Biological Applications | Algae viability assay; microbial growth assays; transketolase activity screening assays; detecting bacteria,g-hydroxybutyric acid (GHB),microorganisms,myocardial infarction; measuring dehydrogenase activity; treating cancer |
| CAS DataBase Reference | 298-96-4(CAS DataBase Reference) |
| EPA Substance Registry System | 2,3,5-Triphenyltetrazolium chloride (298-96-4) |
Safety information for 2,3,5-Triphenyltetrazolium chloride
| Signal word | Danger |
| Pictogram(s) |
 Flame Flammables GHS02  Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H228:Flammable solids H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation |
| Precautionary Statement Codes |
P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P240:Ground/bond container and receiving equipment. P241:Use explosion-proof electrical/ventilating/lighting/…/equipment. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for 2,3,5-Triphenyltetrazolium chloride
| InChIKey | PKDBCJSWQUOKDO-UHFFFAOYSA-M |
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