Tris(hydroxymethyl)aminomethane acetate salt

Synonym(s):TAE buffer;TRIS acetate salt;Tris(hydroxymethyl)aminomethane acetate salt

CAS NO.：6850-28-8
Empirical Formula: C6H15NO5
Molecular Weight: 181.19
MDL number: MFCD00038951
EINECS: 229-939-6
SAFETY DATA SHEET (SDS)
Update Date: 2024-12-18 13:37:16

Tris(hydroxymethyl)aminomethane acetate salt Structural

What is Tris(hydroxymethyl)aminomethane acetate salt?

Chemical properties

White Crystalline Powder

The Uses of Tris(hydroxymethyl)aminomethane acetate salt

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

The Uses of Tris(hydroxymethyl)aminomethane acetate salt

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

What are the applications of Application

Tris acetate salt is a buffer salt used mainly in electrophoresis and chromatography applications

What are the applications of Application

Tris Acetate-EDTA buffer is supplied as a ready to use 1X working solution used for DNA or non-denaturing RNA agarose gel electrophoresis

Definition

ChEBI: An acetate salt resulting from the reaction of equimolar amounts of tris and acetic acid.

Properties of Tris(hydroxymethyl)aminomethane acetate salt

Melting point:	120-121 °C
Boiling point:	219-220 °C (9.7513 mmHg)
Density	1.09 g/mL at 20 °C
storage temp.	Inert atmosphere,Room Temperature
solubility	H₂O: 0.5 M at 20 °C, clear, colorless
form	working solution
color	White
Odor	Acetic odour
PH	6.0-7.0 (25℃, 0.5M in H2O)
PH Range	6 - 7 at 91 g/l at 25 °C
Water Solubility	91 g/l at 20 °C - completely soluble
λmax	λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.05
BRN	3702918
CAS DataBase Reference	6850-28-8(CAS DataBase Reference)
EPA Substance Registry System	1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, acetate (salt) (6850-28-8)

Safety information for Tris(hydroxymethyl)aminomethane acetate salt

Signal word	Warning
Pictogram(s)	Health Hazard GHS08
GHS Hazard Statements	H373:Specific target organ toxicity, repeated exposure
Precautionary Statement Codes	P260:Do not breathe dust/fume/gas/mist/vapours/spray. P314:Get medical advice/attention if you feel unwell. P501:Dispose of contents/container to..…