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HomeProduct name listTriphenylformazan

Triphenylformazan

Synonym(s):TTC formazan

  • CAS NO.:531-52-2
  • Empirical Formula: C19H16N4
  • Molecular Weight: 300.36
  • MDL number: MFCD00020647
  • EINECS: 208-509-1
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2023-04-23 13:52:06
Triphenylformazan Structural

What is Triphenylformazan?

Chemical properties

Dark brown crystalline powder

The Uses of Triphenylformazan

Germination and viability tests.

The Uses of Triphenylformazan

1,3,5-Triphenyltetrazolium formazan has been used:

  • for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
  • as a standard to study the optical density of formazan produced by TTC-treated milled seeds
  • to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

General Description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Properties of Triphenylformazan

Melting point: 169-170 °C
Boiling point: 431.59°C (rough estimate)
Density  1.1454 (rough estimate)
refractive index  1.6140 (estimate)
storage temp.  Store at 2-8
solubility  dioxane: 50 mg/mL, clear
form  powder to crystaline
pka 11.56±0.10(Predicted)
color  Orange to Brown to Dark red
BRN  753057
CAS DataBase Reference 531-52-2(CAS DataBase Reference)
NIST Chemistry Reference Triphenylformazan(531-52-2)
EPA Substance Registry System Diazene, phenyl[phenyl(phenylhydrazono)methyl]- (531-52-2)

Safety information for Triphenylformazan

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
ghs
Environment
GHS09
GHS Hazard Statements H302:Acute toxicity,oral
H400:Hazardous to the aquatic environment, acute hazard
Precautionary Statement Codes P273:Avoid release to the environment.

Computed Descriptors for Triphenylformazan

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