Pyridinium p-Toluenesulfonate

The Uses of Pyridinium p-Toluenesulfonate

Pyridinium p-Toluenesulfonate is used in the synthesis of a subdomain of the cytochrome P450 BM3 enzyme, for use in screening systems for drug candidates.

The Uses of Pyridinium p-Toluenesulfonate

To a flask containing the SM (13.15 g, 52.8 mmol) in dry DCM (90 mL) was added 3,4-dihydro-2H-pyran (10 mL, 110 mmol) followed by pyridinium p-TsOH (0.13 g, 0.53 mmol). The reaction mixture was stirred at a gentle reflux (50 C) for 24 h (monitored by TLC and LCMS). The mixture was concentrated in vacuo. The resulting material was diluted with MeOH and heated in a flask on a rotovap (without vacuum) at 40 C for about 30 min. The mixture was concentrated to about 5 mL in volume, cooled to RT, and filtered. The resulting white solid was rinsed with MeOH (1x) to provide the product. [13.35 g, 76%]