Propidium iodide

Description

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Chemical properties

Dark Red Solid

The Uses of Propidium iodide

1 mg/mL in water

The Uses of Propidium iodide

Antibacterial;Detergent

The Uses of Propidium iodide

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

The Uses of Propidium iodide

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

The Uses of Propidium iodide

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

What are the applications of Application

Propidium iodide solution is a membrane-impermeable fluorescent nucleic acid stain

What are the applications of Application

Propidium Iodide is a membrane-impermeable fluorescent nucleic acid stain that may be used in flow cytometry to evaluate cell viability when used with other dyes.

Definition

ChEBI: Propidium iodide is an organic iodide salt. It contains a propidium.

General Description

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator

storage

+4°C

Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.