Ninhydrin hydrate
Synonym(s):1,2,3-Indantrione monohydrate;2,2-Dihydroxy-1,3-dioxohydrindene, 2,2-Dihydroxy-1,3-indandione, Indantrione hydrate, Triketohydrindene hydrate;2,2-Dihydroxy-1,3-indanedione;Ninhydrin;Trioxohydrindene monohydrate
- CAS NO.:485-47-2
- Empirical Formula: C9H6O4
- Molecular Weight: 178.14
- MDL number: MFCD00003791
- EINECS: 207-618-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2023-11-24 09:23:35
What is Ninhydrin hydrate?
Description
Ninhydrin is historically the most common reagent for processing fingerprints on porous substrates. It has been used as a fingerprint reagent since the 1950s but has been used as a cell stain since the early 1900s. Ninhydrin molecules form a complex with amino acids resulting in a purple product known as Ruhmann's purple. Purple fingerprints are easily visible on light-colored porous substrates but may be difficult to see on patterned or dark-colored substrates. DFO reacts with amino acids to produce a fluorescent product that is not readily seen with the naked eye. This makes it a useful reagent on dark or patterned porous items. DFO prints are viewed with an alternate light source or laser set to 530–570 nm (green light) through a red barrier filter. 1,2-indanedione is an emerging fingerprint reagent that results in visible pink fingerprints that are also fluorescent. The fluorescence is viewed under the same conditions as DFO.
These chemicals can be used on their own or in sequence to develop latent prints on porous substrates. If they are used in sequence, they are used in the following order: indanedione, DFO, ninhydrin. If a reagent is used out of sequence, it will inhibit or limit the effectiveness of the other reagents. In this laboratory exercise you will develop fingerprints on a porous substrate using indanedione, DFO, and ninhydrin.
ninhydrin structure
The Uses of Ninhydrin hydrate
Used for the detection of free amino groups in amino acids, peptides and proteins.
Properties of Ninhydrin hydrate
Melting point: | 250 °C (dec.) (lit.) |
Boiling point: | 230.35°C (rough estimate) |
Density | 0.862 |
Flash point: | 13 °C |
storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
solubility | Soluble in water (50 mg/ml,with sonication), ethanol (100 mg/ml), chloroform (Slightly), ether (Slightly), and methanol (100 mg/ml). |
form | Powder/Solid |
color | white to brownish-white, crystals |
Odor | Odorless |
Water Solubility | 0.1-0.5 g/100 mL at 20 ºC |
Sensitive | Light Sensitive |
Safety information for Ninhydrin hydrate
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for Ninhydrin hydrate
InChIKey | FEMOMIGRRWSMCU-UHFFFAOYSA-N |
Abamectin manufacturer
JAY FINECHEM PVT LTD
SNA HEALTHCARE PVT LTD
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