METHOXYRESORUFIN

Synonym(s):7-Methoxy-3H-phenoxazin-3-one;Methoxyresorufin;O⁷-Methylresorufin

CAS NO.：5725-89-3
Empirical Formula: C13H9NO3
Molecular Weight: 227.22
MDL number: MFCD00037663
SAFETY DATA SHEET (SDS)
Update Date: 2024-07-02 08:55:04

What is METHOXYRESORUFIN?

Description

Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2. Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively. The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity.

The Uses of METHOXYRESORUFIN

Substrate for Cytochrome P-450. METHOXYRESORUFIN is used for various investigations including the differentiatiation of cytochrome P-450 isozymes.

The Uses of METHOXYRESORUFIN

Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).

The Uses of METHOXYRESORUFIN

Used to differentiate isozymes of cytochrome P-450

What are the applications of Application

Resorufin methyl ether is a fluorometric substrate

Biochem/physiol Actions

Fluorimetric substrate for cytochrome P450 linked enzymes.

Properties of METHOXYRESORUFIN

Melting point:	≥220 °C(lit.)
Boiling point:	378.8±42.0 °C(Predicted)
Density	1.33±0.1 g/cm3(Predicted)
storage temp.	2-8°C
solubility	DMF: soluble
pka	1.42±0.20(Predicted)
form	Powder
color	Brown to reddish brown
BRN	209529