Hoechst 33342
Synonym(s):;2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride;bisBenzimide;
- CAS NO.:23491-52-3
- Empirical Formula: C27H28N6O
- Molecular Weight: 452.55
- MDL number: MFCD00166996
- EINECS: 245-691-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-07-02 08:55:42
What is Hoechst 33342?
Chemical properties
yellow powder
The Uses of Hoechst 33342
Hoechst 33342 is useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells.
The Uses of Hoechst 33342
Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342''s fluorescent capability, the
What are the applications of Application
Hoechst 33342, UltraPure grade is a lipophilic fluorescent stain for DNA labeling
What are the applications of Application
Hoechst 33342 trihydrochloride is an A:T-specific DNA minor groove ligand used for visualizing cellular DNA
Definition
ChEBI: 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole is a bibenzimidazole and a N-methylpiperazine. It has a role as a fluorochrome. It is functionally related to a pibenzimol.
Safety Profile
Mutation data reported. Whenheated to decomposition it emits toxic vapors of NOx.
Properties of Hoechst 33342
Boiling point: | 725.9±70.0 °C(Predicted) |
Density | 1.274±0.06 g/cm3(Predicted) |
storage temp. | 2-8°C |
solubility | H2O: soluble20mg/mL |
form | Yellow solid |
pka | 10.97±0.10(Predicted) |
color | Brown to breen |
Water Solubility | Water : 1 mg/mL (2.21 mM; ultrasonic and warming and heat to 80°C) |
BRN | 1234011 |
CAS DataBase Reference | 23491-52-3(CAS DataBase Reference) |
Safety information for Hoechst 33342
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 Health Hazard GHS08 |
GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation H341:Germ cell mutagenicity |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up. |
Computed Descriptors for Hoechst 33342
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