EGS Crosslinker
Synonym(s):Di(N-succinimidyl) ethylene glycol disuccinate;EGS;Ethylene glycol bis(succinimidyl succinate);Ethylene glycol disuccinate di(N-succinimidyl) ester
- CAS NO.:70539-42-3
- Empirical Formula: C18H20N2O12
- Molecular Weight: 456.36
- MDL number: MFCD00049057
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-07-02 08:55:11
What is EGS Crosslinker?
Description
EGS Crosslinker is a cleavable crosslinker which can be cleaved using hydroxyamine. EGS Crosslinkers contain two NHS ester groups which can react with primary amines.
The Uses of EGS Crosslinker
bromoacetate amine and sulfhydryl reactive homobifunctional cross-linking reagent
The Uses of EGS Crosslinker
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is commonly used as a crosslinker in chemical reactions. It is a homobifunctional crosslinking reagent that is reactive with bromoacetate amines and sulfhydryls.
Biochem/physiol Actions
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer.
Properties and Applications
EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS.
Properties of EGS Crosslinker
Boiling point: | 603.4±65.0 °C(Predicted) |
Density | 1.52±0.1 g/cm3(Predicted) |
storage temp. | 2-8°C |
solubility | acetic acid: water (1:1): ≤50mg/mL |
form | powder |
color | White to off-white |
BRN | 9167119 |
InChI | InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2 |
Safety information for EGS Crosslinker
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for EGS Crosslinker
InChIKey | QLHLYJHNOCILIT-UHFFFAOYSA-N |
SMILES | O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O |
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