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HomeProduct name listEGS Crosslinker

EGS Crosslinker

Synonym(s):Di(N-succinimidyl) ethylene glycol disuccinate;EGS;Ethylene glycol bis(succinimidyl succinate);Ethylene glycol disuccinate di(N-succinimidyl) ester

EGS Crosslinker Structural

What is EGS Crosslinker?

Description

EGS Crosslinker is a cleavable crosslinker which can be cleaved using hydroxyamine. EGS Crosslinkers contain two NHS ester groups which can react with primary amines.

The Uses of EGS Crosslinker

bromoacetate amine and sulfhydryl reactive homobifunctional cross-linking reagent

The Uses of EGS Crosslinker

Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is commonly used as a crosslinker in chemical reactions. It is a homobifunctional crosslinking reagent that is reactive with bromoacetate amines and sulfhydryls.

Biochem/physiol Actions

Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer.

Properties and Applications

EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS.

Properties of EGS Crosslinker

Boiling point: 603.4±65.0 °C(Predicted)
Density  1.52±0.1 g/cm3(Predicted)
storage temp.  2-8°C
solubility  acetic acid: water (1:1): ≤50mg/mL
form  powder
color  White to off-white
BRN  9167119
InChI InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2

Safety information for EGS Crosslinker

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P271:Use only outdoors or in a well-ventilated area.
P280:Wear protective gloves/protective clothing/eye protection/face protection.

Computed Descriptors for EGS Crosslinker

InChIKey QLHLYJHNOCILIT-UHFFFAOYSA-N
SMILES O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O

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