Dithizone

Synonym(s):Diphenylthiocarbazone;Dithizone

CAS NO.：60-10-6
Empirical Formula: C13H12N4S
Molecular Weight: 256.33
MDL number: MFCD00003025
EINECS: 200-454-1
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-21 17:11:28

What is Dithizone?

Chemical properties

dark brown or black crystals

The Uses of Dithizone

Dithizone is an organosulfur compound that acts as a chelating agent and forms complexes with metals such as lead and mercury. Dithizone is used to assess the purity of human pancreatic islet preparations used for transplantation into patients with type 1 diabetes.

The Uses of Dithizone

Sensitive reagent for several heavy metals, Co, Cu, Pb, and Hg; especially for the estimation of minute amounts of Pb.

The Uses of Dithizone

Dithizone is used as an indicator for minute amounts of lead. It acts as a chelating agent for heavy metal poisoning. As a ligand, it forms complexes with metals like zinc, lead and mercury. Further, it is used to assess the purity of human pancreatic islet preparations, which is used for transplantation in patients with type 1 diabetes. In addition to this, it is used as a titrant in the quantitative determination of heavy metals.

What are the applications of Application

Dithizone is A titrant in the quantitative determination of heavy metals

Definition

ChEBI: Dithizone is a member of phenylhydrazines.

General Description

Dithizone (Diphenylthiocarbazone) is used as chelating agent for zinc. It is widely used for the liquid-liquid and solid phase extraction of various metals since it forms stable complexes with the metals.

Purification Methods

The crude dithizone is dissolved in CCl4 to give a concentrated solution. This is filtered through a sintered glass funnel and shaken with 0.8M aqueous ammonia to extract dithizonate ion. The aqueous layer is washed with several portions of CCl4 to remove undesirable materials. The aqueous layer is acidified with dilute H2SO4 to precipitate pure dithizone. This is dried in a vacuum. When only small amounts of dithizone are required, purification by paper chromatography is convenient. [Cooper & Hibbits J Am Chem Soc 75 5084 1933.] Instead of CCl4, CHCl3 can be used, and the final extract, after washing with water, can be evaporated in air at 40-50o and dried in a desiccator. It complexes with Cd, Hg, Ni and Zn. [Beilstein 16 H 26, 16 IV 18.]

Properties of Dithizone

Melting point:	168 °C (dec.) (lit.)
Boiling point:	376.1±25.0 °C(Predicted)
Density	1.2578 (rough estimate)
refractive index	1.5700 (estimate)
storage temp.	no restrictions.
solubility	Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
form	Crystalline
pka	pK1:4.50;pK2:15 (25°C)
color	Black powder
Water Solubility	Soluble in ethanol, chloroform, dimethylsulfoxide, and pyridine. Insoluble in water.
Merck	14,3377
BRN	748838
Stability:	Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference	60-10-6(CAS DataBase Reference)
EPA Substance Registry System	Diazenecarbothioic acid, phenyl-, 2-phenylhydrazide (60-10-6)

Safety information for Dithizone

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.