DIHYDROFOLIC ACID

Synonym(s):7,8-Dihydropteroyl-L-glutamic acid;Dihydropteroyl-L-glutamic acid;FAH₂

CAS NO.：4033-27-6
Empirical Formula: C19H21N7O6
Molecular Weight: 443.41
MDL number: MFCD00083619
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-23 13:36:13

What is DIHYDROFOLIC ACID?

The Uses of DIHYDROFOLIC ACID

Dihydrofolic Acid is an intermediate in mammalian conversion of dietary folic acid to tetrahydrofolate by dihydrofolate reductase (DHFR). Folic acid (FA) and Dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF).

The Uses of DIHYDROFOLIC ACID

antidote to methotrexate toxicity

The Uses of DIHYDROFOLIC ACID

What are the applications of Application

Dihydrofolic acid is an intermediate in purine and pyrimidine biosynthesis

Definition

ChEBI: Dihydrofolic acid is a folic acid derivative acted upon by dihydrofolate reductase to produce tetrahydrofolic acid. It interacts with bacteria during cell division and is targeted by various drugs to prevent nucleic acid synthesis. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a conjugate acid of a dihydrofolate(2-).

General Description

Intermediate in mammalian conversion of dietary folic acid to tetrahydrofolate by dihydrofolate reductase (DHFR). In bacteria, dihydrofolic acid is generated from 7,8-dihydropteroate by dihydrofolate synthetase.

Biochem/physiol Actions

Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon′ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).

Purification Methods

DHFA is best purified by suspending (1g mostly dissolved)) in ice-cold sodium ascorbate (300mL of 10% at pH 6.0, prepared by adjusting the pH of 30g of sodium ascorbate in 150mL of H2O by adding 1N NaOH dropwise using a gla

Properties of DIHYDROFOLIC ACID

Melting point:	>160°C (dec.)
Density	1.69±0.1 g/cm3(Predicted)
storage temp.	-20°C
solubility	0.1 M NaOH: 10 mg/mL, slightly hazy, orange
form	Solid
pka	pKa 1.38(H2O t=25 I=0.10) (Occasionally);3.84(H2O t=25 I=0.10) (Occasionally)
color	Light Beige to Beige
BRN	69017
Stability:	Air Sensitive, Hygroscopic, Temperature Sensitive

Safety information for DIHYDROFOLIC ACID

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.