BIOTIN-NHS

Synonym(s):Biotin-NHS;NHS-Biotin;Biotin-OSu;BNHS;N-(Biotinyloxy)succinimide

CAS NO.：35013-72-0
Empirical Formula: C14H19N3O5S
Molecular Weight: 341.38
MDL number: MFCD00078531
EINECS: 609-055-0
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-19 23:02:33

What is BIOTIN-NHS?

Description

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound.

Chemical properties

White Solid

The Uses of BIOTIN-NHS

Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5.

The Uses of BIOTIN-NHS

Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.

The Uses of BIOTIN-NHS

amine reactive biotinylation reagent

Purification Methods

Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.]

Properties of BIOTIN-NHS

Melting point:	212-214 °C
Density	1.45±0.1 g/cm3(Predicted)
storage temp.	-20°C
solubility	DMF: ≤50 mg/mL Stable for at least one month in dry DMF
form	powder
pka	13.89±0.40(Predicted)
color	White to off-white
BRN	4720781
Stability:	Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference	35013-72-0(CAS DataBase Reference)

Safety information for BIOTIN-NHS

Precautionary Statement Codes

P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P271:Use only outdoors or in a well-ventilated area.
P280:Wear protective gloves/protective clothing/eye protection/face protection.