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HomeProduct name listBIOTIN HPDP

BIOTIN HPDP

BIOTIN HPDP Structural

What is BIOTIN HPDP?

Description

Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups. Biotin-HPDP is also used in the biotin switch technique to tag S-nitrosylated (SNO) proteins, following reduction of SNO groups to thiols. Compounds that are tagged with biotin interact avidly with streptavidin-coupled beads, fluorophores, enzymes, etc. The interaction of biotin-HPDP with substrates containing sulfhydryl groups is easily performed at pH 6.5 to 7.5 in buffers such as PBS. The disulfide linkage that is formed between avidin and substrate can later by cleaved by a reducing agent, like dithiothreitol.

Chemical properties

Pale Yellow Solid

The Uses of BIOTIN HPDP

This compounds reacts specifically with thiol groups.

What are the applications of Application

Biotin HPDP is a fully cleavable, biotinylating reagent that reacts specifically with thiol groups.

Properties of BIOTIN HPDP

Melting point: 160-163°C
Boiling point: 876.0±65.0 °C(Predicted)
Density  1.28
storage temp.  -20°C Freezer
solubility  insoluble in H2O; ≥101.4 mg/mL in DMSO; ≥8.29 mg/mL in EtOH with ultrasonic
pka 13.90±0.40(Predicted)
form  powder to crystal
color  White to Light yellow

Safety information for BIOTIN HPDP

Computed Descriptors for BIOTIN HPDP

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