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HomeProduct name listSULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE Structural

What is SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE?

Chemical properties

powder

The Uses of SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

Water soluble biotinylation reagent incorporating an aminocaproyl spacer. This can reduce the steric hindrance in binding avidin to some biotinylated compounds. Has also been used as a cell surface labeling reagent. Typically coupled to primary amine in the pH range 6.5-8.5. May be used for the biotinylation of cell surface proteins.

What are the applications of Application

2′-Deoxy-5′-O-DMT-N6-methyl-8-oxoadenosine 3′-CE phosphoramidite is used in sandwich ELISAs and tumor-site localization

General Description

Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt is a water soluble biotinylation reagent. It can be used in immunoprecipitation of signaling proteins. It can also be used in analysis of hyaluronan synthesis. Further, the product can also be used in ELISA. This biotinylation reagent has shown specificity for human dermal fibroblasts.

Biological Activity

sulfo-nhs-lc-biotin (sulfosuccinimidyl 6-(biotinamido) hexanoate) is an amine-reactive biotinylation agent. as a water-soluble analog of nhs-lc-biotin, sulfo-nhs-lc-biotin contains a negatively charged sulfonate group on the nhs ring structure, which creates sufficient polarity within the molecule and allows it to be directly added into aqueous reactions without prior dissolution of organic solvents. sulfo-nhs-lc-biotin covalently binds to amine groups in proteins and other molecules through its nhs ester forming an amide linkage and releasing n-hydroxysuccinimide. sulfo-nhs-lc-biotin has a greater length (22.4 a) between a covalently modified molecule and the bicyclic biotin rings than nhs-biotin (13.5 a), which provides the probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.

Properties of SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

storage temp.  -20°C
solubility  H2O: soluble10mg/mL
form  powder
color  White to off-white

Safety information for SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

Signal word Danger
Pictogram(s)
ghs
Health Hazard
GHS08
Precautionary Statement Codes P201:Obtain special instructions before use.
P202:Do not handle until all safety precautions have been read and understood.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313:IF exposed or concerned: Get medical advice/attention.
P405:Store locked up.
P501:Dispose of contents/container to..…

Computed Descriptors for SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE

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