6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
- CAS NO.:150810-68-7
- Empirical Formula: C29H25N3O7
- Molecular Weight: 527.53
- MDL number: MFCD07366710
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-07-02 08:55:00
What is 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER?
Description
5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin , nucleotide diphosphate uridine-5’-diphosphate (UDP; ), and the progesterone receptor antagonist RU486 (mifepristone; ). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.
The Uses of 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
Amine-reactive form of 5-carboxytetramethylrhodamine single isomer.
The Uses of 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
Amine-Reactive Fluorescent Probe for labeling of DNA and RNA
What are the applications of Application
5-Carboxy-tetramethylrhodamine N-succinimidyl ester is a chemical used in fluorescent labeling of DNA and RNA
storage
Store at -20°C
Properties of 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
storage temp. | Keep in dark place,Inert atmosphere,Store in freezer, under -20°C |
solubility | DMF: soluble |
form | A solid |
color | Pale purple to purple |
Safety information for 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H302:Acute toxicity,oral H317:Sensitisation, Skin |
Precautionary Statement Codes |
P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
InChIKey | VWFRSNKRTNUMET-UHFFFAOYSA-N |
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