5(6)-Carboxyfluorescein
Synonym(s):5(6)-Carboxyfluorescein;5(6)-FAM;5,6-CF
- CAS NO.:72088-94-9
- Empirical Formula: C42H24O14
- Molecular Weight: 752.63
- MDL number: MFCD00151081
- EINECS: 276-331-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-18 17:01:59
What is 5(6)-Carboxyfluorescein?
Description
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
Chemical properties
Dark-Orange to Brown Solid
The Uses of 5(6)-Carboxyfluorescein
As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
The Uses of 5(6)-Carboxyfluorescein
A popular probe of determination of intracellular pH.
The Uses of 5(6)-Carboxyfluorescein
5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
What are the applications of Application
5(6)-Carboxyfluorescein is used in the preparation of fluorescent peptide and protein conjugates
What are the applications of Application
5(6)-carboxyfluorescein, mixed isomers is a pH indicator
Definition
ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.
Solubility in organics
5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.
Properties of 5(6)-Carboxyfluorescein
Melting point: | 275 °C |
Boiling point: | 464.9°C (rough estimate) |
Density | 1.3418 (rough estimate) |
refractive index | 1.6380 (estimate) |
storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
form | Solid |
color | Light Yellow to Dark Brown |
Water Solubility | slightly soluble |
EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
Safety information for 5(6)-Carboxyfluorescein
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for 5(6)-Carboxyfluorescein
InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 |
New Products
4-Aminotetrahydropyran-4-carbonitrile Hydrochloride (R)-3-Aminobutanenitrile Hydrochloride 4-AMINO-TETRAHYDRO-PYRAN-4-CARBOXYLIC ACID HCL 4-(Dimethylamino)tetrahydro-2H-pyran-4-carbonitrile 3-((Dimethylamino)methyl)-5-methylhexan-2-one oxalate 1,4-Dioxa-8-azaspiro[4.5]decane 5-Bromo-2-nitropyridine Nimesulide BP Aceclofenac IP/BP/EP Diclofenac Sodium IP/BP/EP/USP Mefenamic Acid IP/BP/EP/USP Ornidazole IP Diclofenac Potassium SODIUM AAS SOLUTION ZINC AAS SOLUTION BUFFER SOLUTION PH 10.0(BORATE) GOOCH CRUCIBLE SINTERED AQUANIL 5 BERYLLIUM AAS SOLUTION 2-Bromo-1-(bromomethyl)-3-chloro-5-nitrobenzene 2-Bromo-3-nitroaniline N-(3-Hydroxypropyl)-N-methylacetamide 3-Bromo-6-chloropyridazine 4-ethyl-3-nitrobenzoic acidRelated products of tetrahydrofuran
You may like
-
5(6)-Carboxyfluorescein, mixture of isomers CAS 72088-94-9View Details
72088-94-9 -
5(6)-Carboxyfluorescein CAS 72088-94-9View Details
72088-94-9 -
5(6)-Carboxyfluorescein CAS 72088-94-9View Details
72088-94-9 -
5(6)-Carboxyfluorescein CAS 72088-94-9View Details
72088-94-9 -
1823368-42-8 98%View Details
1823368-42-8 -
2-(3-(tert-butyl)phenoxy)-2-methylpropanoic acid 1307449-08-6 98%View Details
1307449-08-6 -
Ethyl 3-(furan-2-yl)-3-hydroxypropanoate 25408-95-1 98%View Details
25408-95-1 -
Lithium ClavulanateView Details
61177-44-4