Thiazolyl Blue
Synonym(s):3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue;Methylthiazolyldiphenyl-tetrazolium bromide;MTT;Thiazolyl blue, MTT
- CAS NO.:298-93-1
- Empirical Formula: C18H16BrN5S
- Molecular Weight: 414.32
- MDL number: MFCD00011964
- EINECS: 206-069-5
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-12-18 14:07:02
What is Thiazolyl Blue?
Chemical properties
Thiazolyl Blue Tetrazolium Blue (MTT) is a water soluble tetrazolium salt yielding a yellowish solution when prepared in media or salt solutions lacking phenol red. Dissolved MTT is converted to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water insoluble formazan can be solubilized using isopropanol or other solvents and the dissolved material is measured spectrophotometrically yielding absorbance as a function of concentration of converted dye.
The Uses of Thiazolyl Blue
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
What are the applications of Application
Thiazolyl Blue may be used in measurement of cell proliferation and is a membrane-permeable dye reduced by mitochondrial enzymes
Definition
ChEBI: Thiazolyl Blue is the bromide salt of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. It has a role as a dye and a colorimetric reagent. It contains a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium.
General Description
Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. It is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells. As an electron acceptor, it is utilized for studying nicotinamide adenine dinucleotide phosphate (NADP)-dependent dehydrogenases.
Biochem/physiol Actions
Cell permeable: yes
storage
+4°C
Purification Methods
It is recrystallised by dissolving in MeOH containing a few drops of HBr and then adding dry Et2O to complete the crystallisation, wash the needles with Et2O and dry them in a vacuum desiccator over KOH. [Beyer & Pyl Chem Ber 87 1505 1954, Beilstein 27 III/IV 6045.]
Properties of Thiazolyl Blue
Melting point: | 195 °C (dec.) (lit.) |
Density | 1.4886 (estimate) |
storage temp. | 2-8°C |
solubility | methanol: 5 mg/mL, clear |
form | powder |
color | Yellow to orange |
Water Solubility | Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing |
λmax | 378 nm, 242 nm |
Sensitive | Light Sensitive |
BRN | 3825277 |
Stability: | Hygroscopic |
Biological Applications | Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer |
CAS DataBase Reference | 298-93-1(CAS DataBase Reference) |
EPA Substance Registry System | 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1) |
Safety information for Thiazolyl Blue
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 Health Hazard GHS08 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation H341:Germ cell mutagenicity |
Precautionary Statement Codes |
P201:Obtain special instructions before use. P202:Do not handle until all safety precautions have been read and understood. P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P308+P313:IF exposed or concerned: Get medical advice/attention. |
Computed Descriptors for Thiazolyl Blue
InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
Thiazolyl Blue manufacturer
Neugen Labs
Anand Agencies
Neugen Labs
New Products
(S)-3-Aminobutanenitrile hydrochloride 4-Methylphenylacetic acid N-Boc-D-alaninol N-BOC-D/L-ALANINOL Tert-butyl bis(2-chloroethyl)carbamate 3-Morpholino-1-(4-nitrophenyl)-5,6-dihydropyridin- 2(1H)-one Furan-2,5-Dicarboxylic Acid Tropic acid 1-Bromo-3,5-Di-Tert-Butylbenzene S-2-CHLORO PROPIONIC ACID ETHYL ISOCYANOACETATE 2-Bromo-1,3-Bis(Dimethylamino)Trimethinium Hexafluorophosphate 4-IODO BENZOIC ACID 3-NITRO-2-METHYL ANILINE 1-(2,4-DICHLOROPHENYL) ETHANAMINE (2-Hydroxyphenyl)acetonitrile 4-Bromopyrazole 2-(Cyanocyclohexyl)acetic acid 4-methoxy-3,5-dinitropyridine 1-(4-(aminomethyl)benzyl)urea hydrochloride 2-aminopropyl benzoate hydrochloride diethyl 2-(2-((tertbutoxycarbonyl)amino) ethyl)malonate tert-butyl 4- (ureidomethyl)benzylcarbamate Ethyl-2-chloro((4-methoxyphenyl)hydrazono)acetateRelated products of tetrahydrofuran
You may like
-
Thiazolyl Blue tetrazolium bromide CAS 298-93-1View Details
298-93-1 -
Thiazolyl Blue Tetrazolium Bromide (MTT) for tissue culture CAS 298-93-1View Details
298-93-1 -
Ethyl methacrylate 95% CAS 298-93-1View Details
298-93-1 -
Thiazolyl blue tetrazolium bromide 98% (HPLC) CAS 298-93-1View Details
298-93-1 -
MTT tetrazolium, suitable for cell culture CAS 298-93-1View Details
298-93-1 -
MTT tetrazolium CAS 298-93-1View Details
298-93-1 -
MTT [for Biochemical Research] CAS 298-93-1View Details
298-93-1 -
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide CAS 298-93-1View Details
298-93-1