Solvent Black 3
Synonym(s):Ceres black BN;Fat Black HB;Solvent Black 3;Sudan Black B Powder
- CAS NO.:4197-25-5
- Empirical Formula: C29H24N6
- Molecular Weight: 456.54
- MDL number: MFCD00006919
- EINECS: 224-087-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-12-18 14:08:57
What is Solvent Black 3?
Chemical properties
It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.
The Uses of Solvent Black 3
Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.
What are the applications of Application
Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids
Definition
ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.
Preparation
aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.
Safety Profile
A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.
Properties and Applications
Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.
Standard | Light Fastness | Heat-resistant(℃) | water | Sodium Carbonate(5%) | Hydrochloric acid(5%) | |
Melting point | Stable | |||||
ISO | General | 126 | 100 | Insoluble | No change | No change |
Properties of Solvent Black 3
Melting point: | 120-124 °C(lit.) |
Boiling point: | 552.68°C (rough estimate) |
Density | 1.4899 (rough estimate) |
refractive index | 1.4570 (estimate) |
storage temp. | Store at RT. |
solubility | Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol |
form | Powder/Solid |
pka | 2.94±0.40(Predicted) |
Colour Index | 26150 |
color | Very dark brown to black |
Water Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. |
λmax | 598 nm, 415 nm |
Merck | 13,8970 |
BRN | 723248 |
Stability: | Light Sensitive |
Biological Applications | Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening |
CAS DataBase Reference | 4197-25-5(CAS DataBase Reference) |
EPA Substance Registry System | C.I. Solvent Black 3 (4197-25-5) |
Safety information for Solvent Black 3
Signal word | Warning |
Pictogram(s) |
Health Hazard GHS08 |
GHS Hazard Statements |
H341:Germ cell mutagenicity |
Precautionary Statement Codes |
P201:Obtain special instructions before use. P202:Do not handle until all safety precautions have been read and understood. P280:Wear protective gloves/protective clothing/eye protection/face protection. P308+P313:IF exposed or concerned: Get medical advice/attention. P405:Store locked up. P501:Dispose of contents/container to..… |
Computed Descriptors for Solvent Black 3
Solvent Black 3 manufacturer
Vikram And Company
ARRAKIS INDUSTRIES LLP
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