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HomeProduct name listSolvent Black 3

Solvent Black 3

Synonym(s):Ceres black BN;Fat Black HB;Solvent Black 3;Sudan Black B Powder

  • CAS NO.:4197-25-5
  • Empirical Formula: C29H24N6
  • Molecular Weight: 456.54
  • MDL number: MFCD00006919
  • EINECS: 224-087-1
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-07-31 17:39:26
Solvent Black 3 Structural

What is Solvent Black 3?

Chemical properties

It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.

The Uses of Solvent Black 3

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

What are the applications of Application

Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids

Definition

ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Standard Light Fastness Heat-resistant(℃) water Sodium Carbonate(5%) Hydrochloric acid(5%)
Melting point Stable
ISO General 126 100 Insoluble No change No change

Properties of Solvent Black 3

Melting point: 120-124 °C(lit.)
Boiling point: 552.68°C (rough estimate)
Density  1.4899 (rough estimate)
refractive index  1.4570 (estimate)
storage temp.  Store at RT.
solubility  Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol
form  Powder/Solid
pka 2.94±0.40(Predicted)
Colour Index  26150
color  Very dark brown to black
Water Solubility  Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.
λmax 598 nm, 415 nm
Merck  13,8970
BRN  723248
Stability: Light Sensitive
Biological Applications Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening
CAS DataBase Reference 4197-25-5(CAS DataBase Reference)
EPA Substance Registry System C.I. Solvent Black 3 (4197-25-5)

Safety information for Solvent Black 3

Signal word Warning
Pictogram(s)
ghs
Health Hazard
GHS08
GHS Hazard Statements H341:Germ cell mutagenicity
Precautionary Statement Codes P201:Obtain special instructions before use.
P202:Do not handle until all safety precautions have been read and understood.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313:IF exposed or concerned: Get medical advice/attention.
P405:Store locked up.
P501:Dispose of contents/container to..…

Computed Descriptors for Solvent Black 3

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