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HomeProduct name listSolvent Red 27

Solvent Red 27

Synonym(s):1-([4-(Xylylazo)xylyl]azo)-2-naphthol;1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol;Solvent Red 27;Sudan Red 5B

  • CAS NO.:1320-06-5
  • Empirical Formula: C26H24N4O
  • Molecular Weight: 408.49
  • MDL number: MFCD00003898
  • EINECS: 215-295-3
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2023-05-04 17:34:32
Solvent Red 27 Structural

What is Solvent Red 27?

Physical properties

Solvent Red 27 is a dark green to dark brown crystalline powder.

The Uses of Solvent Red 27

Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.

The Uses of Solvent Red 27

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.

What are the applications of Application

Oil Red O is an agent that stains lipids, muscle fibers and alveolar macrophages

Preparation

2-Amino-2′,3,4′,5-tetramethylazobenzene diazotization, and Naphthalen-2-ol coupling.

Definition

ChEBI: Oil red O is a bis(azo) compound that is 2-naphthol substituted at position 1 by a {4-[(2,5-dimethylphenyl)diazenyl]-2,5-dimethylphenyl}diazenyl group. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of naphthols. It is functionally related to a 2-naphthol.

General Description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Properties and Applications

blue light red. Soluble in ethanol for yellow red, soluble in acetone, soluble in benzene. In concentrated sulfuric acid to green light blue, blue purple precipitation after dilution.

Standard Light Fastness Heat-resistant(℃) water Sodium Carbonate(5%) Hydrochloric acid(5%)
Melting point Stable
ISO Well 108-120 70≥ 200

Sublimation

Insoluble No change No change

Properties of Solvent Red 27

Melting point: 120 °C (dec.)(lit.)
Flash point: 103℃
storage temp.  room temp
solubility  chloroform/ethanol (1:1): soluble1mg/mL
form  powder
Colour Index  26125
color  Red to dark greenish-brown
Water Solubility  Soluble in ethanol:chloroform (1:1). Insoluble in water.
λmax 518 nm, 359 nm
BRN  3491382
Biological Applications Detecting early stages of atherosclerotic lesion formation; treating obesity,hyperlipidemia,fatty liver,leptin production disorders; dental impression material; measuring phagocytosis
CAS DataBase Reference 1320-06-5(CAS DataBase Reference)
EPA Substance Registry System C.I. Solvent Red 27 (1320-06-5)

Safety information for Solvent Red 27

Signal word Danger
Pictogram(s)
ghs
Flame
Flammables
GHS02
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H225:Flammable liquids
H319:Serious eye damage/eye irritation
H336:Specific target organ toxicity,single exposure; Narcotic effects
Precautionary Statement Codes P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for Solvent Red 27

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