Hoechst 33258

Chemical properties

yellow to yellow-green powder

The Uses of Hoechst 33258

Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence

The Uses of Hoechst 33258

Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.

The Uses of Hoechst 33258

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =?346 nm
Emission max. =?460 nm

What are the applications of Application

Hoechst 33258 is a fluorescent stain used for labeling DNA in fluorescent microscopy

What are the applications of Application

An A-T specific highly fluorescent DNA stain that has many uses

What are the applications of Application

Hoechst 33258, UltraPure grade is a fluorescent stain useful for DNA labeling

General Description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

storage

+4°C (desiccate)