Hematoxylin

Chemical properties

light brown crystals or powder

The Uses of Hematoxylin

Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.

The Uses of Hematoxylin

Colorant in inks, biological stains.

What are the applications of Application

Gill′s Hematoxylin Solution, No. 3 is a nuclei stain used in histology

What are the applications of Application

Gill′s Hematoxylin Solution, No. 1 is a chromatin stain used in cytology

What are the applications of Application

Gill′s Hematoxylin Solution, No. 2 is a chromatin stain of blue-violet color used for both cytology and histology

Definition

ChEBI: (+)-haematoxylin is a haematoxylin. It is an enantiomer of a (-)-haematoxylin.

General Description

White to yellowish crystals that redden on exposure to light.

Air & Water Reactions

Soluble in hot water, slightly water soluble in cold .

Reactivity Profile

Sensitive to light. .

Hazard

May be carcinogenic.

storage

Store at RT

Purification Methods

Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.]