Hematoxylin

Synonym(s):H&E Stain, Gill;Hematoxylin;Hematoxylin cryst. (C.I. 75290);Natural Black 1

CAS NO.：517-28-2
Empirical Formula: C16H14O6
Molecular Weight: 302.28
MDL number: MFCD00283323
EINECS: 208-237-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-01 18:09:03

What is Hematoxylin?

Chemical properties

light brown crystals or powder

The Uses of Hematoxylin

Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.

The Uses of Hematoxylin

Colorant in inks, biological stains.

Definition

ChEBI: (+)-haematoxylin is a haematoxylin. It is an enantiomer of a (-)-haematoxylin.

What are the applications of Application

Gill′s Hematoxylin Solution, No. 3 is a nuclei stain used in histology

What are the applications of Application

Gill′s Hematoxylin Solution, No. 1 is a chromatin stain used in cytology

What are the applications of Application

Gill′s Hematoxylin Solution, No. 2 is a chromatin stain of blue-violet color used for both cytology and histology

General Description

White to yellowish crystals that redden on exposure to light.

Air & Water Reactions

Soluble in hot water, slightly water soluble in cold .

Reactivity Profile

Sensitive to light. .

Hazard

May be carcinogenic.

storage

Store at RT

Purification Methods

Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.]

Properties of Hematoxylin

Melting point:	200 °C (dec.)(lit.)
Boiling point:	363.32°C (rough estimate)
Density	1.2514 (rough estimate)
vapor pressure	0-0Pa at 20-25℃
refractive index	1.4600 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
solubility	Soluble in 95% ethanol(1 mg/mL).
form	Powder/Solid
Colour Index	75290
pka	6.7(at 25℃)
color	Yellow to Brown
PH Range	Red (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0)
Water Solubility	SOLUBLE IN HOT WATER
Sensitive	Light Sensitive
λmax	292nm, 445nm, 560nm
Merck	14,4637
BRN	91399
Stability:	Stable, but may discolour on exposure to light. Incompatible with strong oxidizing agents.
Major Application	Plasma displays, textiles, hair dyes, identifying fresh and stale rice, diag-nosing cancer progression, detecting nosing cancer progression, cervical disease, central nervous system malfunctions, detecting genes, breast cancer, collagen in a tissue sample, apoptosis, demyelinating diseases, antigens, treatment of age-related macular degeneration, burns, prostate cancer, diabetesand obesity, viral diseases, neoplasms, peripheral neural and vascular ailments, skin disorders, biotechnological applications, reference standard materials for cytology, histology andimmunohistochemistry
CAS DataBase Reference	517-28-2
EPA Substance Registry System	Hematoxylin (517-28-2)

Safety information for Hematoxylin

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P337+P313:IF eye irritation persists: Get medical advice/attention.