Glucose oxidase

Description

Produced by the controlled fermentation of Aspergillus niger var. as a yellow to brown solution or as a yellow to tan or off-white powder. Soluble in water, the solutions being light yellow to brown, but practically insoluble in alcohol, chloroform or ether. The major active principle is glucose oxidase, IUB No. 1.1.3.4, CAS No. 9001-37-0.

Chemical properties

yellow powder

The Uses of Glucose oxidase

glucose oxidase is an enzyme used to help stabilize a formulation. It can also improve skin feel and condition.

The Uses of Glucose oxidase

Analytical reagent for the selective determination of glucose. Food additive for the removal of glucose during the preparation of dried egg products. Antioxidant in food and food wrappers. Stabilizer for ascorbic acid and vitamin B12.

The Uses of Glucose oxidase

Glucose Oxidase from Aspergillus niger has been used:

• in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method
• to activate the human renal carcinoma cell line for constructing the oxidative stress model
• to study its influence in the paste on the analytical performance of the bioelectrode

Definition

An enzyme commercially available under various trademarks; catalyzes oxidation of glucose to gluconic acid. Water-soluble amorphous powder. Removes excess oxygen from canned foods, beer, etc., and from stored food.

General Description

Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175?kDa. Some fungi and insects are capable of producing GOX.

Flammability and Explosibility

Not classified

Industrial uses

In nature, the enzymes glucose oxidase (GOx) or glucose dehydrogenase (GDH) are used as biosensors for blood glucose monitors.

Biochem/physiol Actions

Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.

Purification Methods

The oxidase is purified by dialysis against deionized water at 6o for 48hours and by molecular exclusion chromatography with Sephadex G-25 at room temperature. [Holt & Cotton J Am Chem Soc 109 1841 1987.]