FMoc-NH-5(ethylene glycol)-acetic acid

CAS NO.：635287-26-2
Empirical Formula: C27H35NO9
Molecular Weight: 517.57
MDL number: MFCD26142982
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-23 13:36:13

What is FMoc-NH-5(ethylene glycol)-acetic acid?

Description

Fmoc-NH-PEG5-CH2COOH is a PEG linker containing an Fmoc-protected amine and a terminal carboxylic acid. The hydrophilic PEG spacer increases solubility in aqueous media. The Fmoc group can be deprotected under basic condition to obtain the free amine which can be used for further conjugations. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond.

Biological Activity

Fmoc-NH-PEG5-CH2COOH is a degradable ADC linker for antibody drug conjugate (ADC) synthesis. It is also a PEG-based PROTAC linker, which can be used for PROTAC synthesis.

in vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.

Properties of FMoc-NH-5(ethylene glycol)-acetic acid

Boiling point:	704.3±60.0 °C(Predicted)
Density	1.224±0.06 g/cm3(Predicted)
storage temp.	2-8°C
pka	3.39±0.10(Predicted)
form	Viscous Liquid
color	Colorless to light yellow