Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

Synonym(s):2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt;2,2′-azino-di-[3-ethylbenzthiazoline sulfonate (6)];AzBTS-(NH₄)₂;azino-di-[3-ethylbenzthiazoline sulfonate (6)], 2,2′-;Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

CAS NO.：30931-67-0
Empirical Formula: C18H24N6O6S4
Molecular Weight: 548.68
MDL number: MFCD00010404
EINECS: 250-396-6
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-04 20:04:50

Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Structural

What is Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)?

Chemical properties

Light green powder

The Uses of Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

Diammonium-2,2’Azino-bis(3-ethylbenzothiazoline-6-sulfonate) has been shown to inhibit catalase, a mammalian antioxidant enzyme which degrades hydrogen peroxide into water and oxygen species.

The Uses of Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

Chromogenic substrate for peroxidase assays.2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt is widely utilized as a peroxidase substrate suitable for use in enzyme-linked immunosorbent assay (ELISA) procedures. It plays an important role in the food industry to measure the antioxidant capacities of foods. It is used to observe the reaction kinetics of specific enzymes and involved in glucose estimating reagent in blood serum.

The Uses of Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).?
ABTS is also used to measure glucose concentrations in different solutions including blood serum.
The food industry uses ABTS to measure the antioxidant capacities within different foods.
Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

What are the applications of Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is a peroxidase ELISA substrate

General Description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:

ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).??? ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

Properties of Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

Melting point:	>181oC (dec.)
storage temp.	2-8°C
solubility	H₂O: 50 mg/mL, very slightly hazy, green
form	tablet
color	Pale Green to Light Green
PH	pH(50g/l, 25℃) : 5.0～6.0
Water Solubility	Dissolve in water at 50mg/ml. Solution may be slightly hazy.
Sensitive	Light Sensitive
BRN	7329461
Stability:	Hygroscopic, Light Sensitive
CAS DataBase Reference	30931-67-0(CAS DataBase Reference)

Safety information for Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up.