DC-CHOL

Synonym(s):3β-[N-(Dimethylaminoethane)-carbamoyl]-cholesterol;3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride;DC-Chol

CAS NO.：166023-21-8
Empirical Formula: C32H56N2O2.ClH
Molecular Weight: 537.26
MDL number: MFCD00467173
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-16 15:32:52

What is DC-CHOL?

Description

DC-Chol is a cationic cholesterol derivative. DC-Chol, as a component of lipoplexes with DOPE , has been used for transfection of mRNA into A549 cells without affecting cell viability. Incubation of DC-chol/DOPE liposomes or lipoplexes with human whole blood has no effect on neutrophil elastase or β-thromboglobulin levels or the number of platelets and red and white blood cells, indicating hemocompatibility. DC-Chol has also been widely used in the synthesis of liposomes for the delivery of siRNA, DNA, and chemotherapeutic agents into cells and mice.

The Uses of DC-CHOL

DC-Cholesterol HCl is suitable:

for the preparation of ultrasound cationic microbubbles
to prepare liposomes
for the preparation of L-glutathione (GSH), loaded proliposomes

The Uses of DC-CHOL

This cationic cholesterol derivative forms liposomes with L-α-phosphatidylethanolamine, dioleoyl, that efficiently transfect mammalian cell lines.

What are the applications of Application

DC-Cholesterol is a reagent for mammalian cell transfection

General Description

DC-Cholesterol HCl is a sterol-based transfection reagent.

Biochem/physiol Actions

DC-Cholesterol is an effective transfection agent and a liposome reagent. Liposome of DC-Cholesterol with dioleoylphosphatidylethanolamine (DOPE) are efficient for DNA transfection. It imparts positive surface charge to the microbubbles for ultra sound based transfection of clustered regularly interspaced short palindromic repeats (CRISPRs).