DBCO-PEG4-amine

CAS NO.：1255942-08-5
Empirical Formula: C29H37N3O6
Molecular Weight: 523.62
MDL number: MFCD22380748
Update Date: 2024-03-28 13:39:29

What is DBCO-PEG4-amine?

Description

DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions.

Biological Activity

DBCO-NHCO-PEG4-amine is a PROTAC linker and belongs to the PEG class. It is a cleavable ADC linker that can be used to link MMAE (HY-15162) to antibodies such as DBCO-VCpAB-MMAE and DBCO-TRX-MMAE, with EC50 for SKBR3 cells, respectively 280 nM and 22 nM).

in vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

Properties of DBCO-PEG4-amine

Boiling point:	784.1±60.0 °C(Predicted)
Density	1.23±0.1 g/cm3(Predicted)
storage temp.	-20°C Freezer, Under inert atmosphere
solubility	Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
form	Solid
pka	15.14±0.46(Predicted)
color	Yellow to Dark Yellow Sticky
Water Solubility	Soluble in water.

Safety information for DBCO-PEG4-amine

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up.