D-Luciferin potassium salt
Synonym(s):(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid potassium salt;4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt;Firefly luciferin potassium salt
- CAS NO.:115144-35-9
- Empirical Formula: C11H9KN2O3S2
- Molecular Weight: 320.42
- MDL number: MFCD00044928
- EINECS: 1592732-453-0
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 23:02:33
What is D-Luciferin potassium salt ?
Description
D-Luciferin potassium salt(115144-35-9) is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by lucgene.
Chemical properties
Yellow powder
The Uses of D-Luciferin potassium salt
(4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic Acid Monopotassium Salt, is a class of light-emitting heterocyclic compounds found in organisms that cause bioluminescence, such as firefly. It produces light upon oxidative decarboxylation by luciferase in the presence of ATP.
What are the applications of Application
D-Luciferin potassium salt is a light-emitting heterocycle compounds found in organisms that cause bioluminescence, such as firefly
What are the applications of Application
D-Luciferin may be used as a substrate in luciferase-based (EC 1.13.12.7) bioluminescence imaging and cell-based high-throughput screening applications. ATP can be measured with a reagent made up of D-luciferin and luciferase from firefly. D-Luciferin may be used and studied as a partial agonist for G protein-coupled receptor-35 (GPR35). Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.
Properties of D-Luciferin potassium salt
storage temp. | Inert atmosphere,Store in freezer, under -20°C |
solubility | DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM) DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM) Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM) PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM) Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM) |
form | A crystalline solid |
color | Light yellow |
BRN | 5416262 |
InChI | InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3 |
CAS DataBase Reference | 115144-35-9(CAS DataBase Reference) |
Safety information for D-Luciferin potassium salt
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for D-Luciferin potassium salt
InChIKey | UMBKGTQQGYPQBE-OGFXRTJISA-M |
SMILES | C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r| |
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