Coumarin 120

Chemical properties

Coumarin 120 is Light yellow beige powder

The Uses of Coumarin 120

suitable for determining polygalacturonase activity

The Uses of Coumarin 120

λ max 354 nm in EthanolSuitable as laser dye and used as a fluorescent labeling reagent for trace determination of enzymes.

The Uses of Coumarin 120

Coumarin 120 is widely used fluorophore, well-known for preparing fluorogenic substrates for cystine aminopeptidase and other hydrolases. Used as reference compound in enzyme assays.

What are the applications of Application

7-Amino-4-methylcoumarin is a fluorogenic reagent and antibacterial, antifungal agent

Definition

ChEBI: Coumarin 120 is a member of 7-aminocoumarins. It has a role as a fluorochrome.

General Description

7-Amino-4-methylcoumarin (coumarin120) is an aminocoumarin laser dye that can emit laser in the range of 370-760 nm. It has three intermolecular hydrogen bonded structures with C-H···O and N-H····O.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

storage

+4°C

Purification Methods

Dissolve it in 5% HCl, filter and basify with 2M ammonia. The precipitate is dried in a vacuum and recrystallised from dilute EtOH. It yields a blue solution and is light sensitive. [Sigler Synthesis 614 1980, Kanaoka et al. Chem Pharm Bull Jpn 30 1485 1982, Beilstein 18/11 V 445.]