Coumarin 120

Chemical properties

Coumarin 120 is Light yellow beige powder

The Uses of Coumarin 120

Coumarin 120 is widely used fluorophore, well-known for preparing fluorogenic substrates for cystine aminopeptidase and other hydrolases. Used as reference compound in enzyme assays.

The Uses of Coumarin 120

suitable for determining polygalacturonase activity

The Uses of Coumarin 120

λ max 354 nm in EthanolSuitable as laser dye and used as a fluorescent labeling reagent for trace determination of enzymes.

What are the applications of Application

7-Amino-4-methylcoumarin is a fluorogenic reagent and antibacterial, antifungal agent

Definition

ChEBI: Coumarin 120 is a member of 7-aminocoumarins. It has a role as a fluorochrome.

General Description

7-Amino-4-methylcoumarin (coumarin120) is an aminocoumarin laser dye that can emit laser in the range of 370-760 nm. It has three intermolecular hydrogen bonded structures with C-H···O and N-H····O.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

storage

+4°C

Purification Methods

Dissolve it in 5% HCl, filter and basify with 2M ammonia. The precipitate is dried in a vacuum and recrystallised from dilute EtOH. It yields a blue solution and is light sensitive. [Sigler Synthesis 614 1980, Kanaoka et al. Chem Pharm Bull Jpn 30 1485 1982, Beilstein 18/11 V 445.]