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HomeProduct name listCI 51180

CI 51180

Synonym(s):Basic Blue 12;Nile blue sulfate

  • CAS NO.:3625-57-8
  • Empirical Formula: C20H20N3O5S-
  • Molecular Weight: 414.46
  • MDL number: MFCD00064529
  • EINECS: 222-832-5
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-07-15 17:50:29
CI 51180 Structural

What is CI 51180?

Chemical properties

dark green to black powder

The Uses of CI 51180

Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

The Uses of CI 51180

Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.

The Uses of CI 51180

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

What are the applications of Application

Nile blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

General Description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Purification Methods

Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]

Properties of CI 51180

Melting point: >300 °C (dec.)(lit.)
storage temp.  room temp
solubility  Soluble in water, ethanol
Colour Index  51180
form  Powder
color  Dark green to black
PH Range 10.1(BLUE)-11.1(RED)
Water Solubility  Soluble in ethanol, and water (50 g/L) at 25°C.
λmax 633 nm
Biological Applications Detecting microorganisms; treating virus infectious diseases; photodynamic therapy

Safety information for CI 51180

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for CI 51180

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